*Dear all,* *We are looking for a structural biologist/biochemist for structural studies of bacterial chromosome/plasmid segregation machinery. Please see the advert below, and bring to the attention of anyone in your labs who may be interested. Thank you very much.*
*Best wishes,* *Tung Le* *We are looking for a structural biologist/biochemist to join a 5-year Wellcome Trust-funded project in the Le lab (John Innes Centre-Department of Molecular Microbiology-UK **https://www.tunglelab.org/* <http://www.tunglelab.org>*).* The chromosomes of all living organisms must be compacted nearly three orders of magnitude to fit within cells. Moreover, DNA must be packaged in a way that is compatible with a myriad of DNA-based processes, including replication, segregation, transcription, repair, and recombination. This challenge is particularly acute in bacteria as chromosome segregation occurs concomitantly with DNA replication rather than being separated temporally, as in eukaryotes. How chromosome organization and its integration with biological processes are achieved in vivo remains poorly understood. To fill this knowledge gap, our lab aims to: 1) Elucidate the molecular mechanisms underlying chromosome organization and segregation 2) Unravel the relationship between spatial chromosome organization (locally and globally) and important biological processes in the cell. We are particularly interested in how the Structural Maintenance of Chromosomes (SMC) protein and Partitioning proteins (ParA-ParB-*parS*) interact with DNA to organize and segregate the bacterial chromosome. The combination of structural biology, genetics, cell biology, and genome-wide techniques (ChIP-seq and Hi-C) has proved to be very effective in investigating SMC/ParB in our lab. In the long term, we aim to apply similar approaches to investigate other classes of chromosome-structuring proteins. The ultimate aim of our lab is to understand the integration of actions of different classes of architecture proteins in organizing the chromosome. The candidate will join a diverse group of students, postdocs, and scientists and work closely with Prof. David Lawson, the John Innes Centre (JIC) X-ray crystallography platform manager ( https://www.jic.ac.uk/research-impact/technology-research-platforms/protein-crystallography/). The JIC also has a FEI Talos F200C TEM for sample screening purposes. Candidates willing to learn cryo-EM is welcomed. .*Qualifications:* · PhD in a relevant field · Experience in protein purification and enzymology · Experience in X-ray crystallography or cryo-EM. · Experience with microbiology is desirable · Experience with AFM or HDX-MS is desirable · Organized and detail-oriented · Enjoys collaborations and teamwork. *To apply:* Email tung...@jic.ac.uk with · a cover letter that highlights your experience, contributions to science, and interests · CV We prioritize inclusiveness in our team and encourage individuals from diverse backgrounds, and individuals committed to promoting diversity, equity, and inclusion, to apply. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
