Jürgen Quote: "Propane for whatever reason has gone extinct in certain
areas of the world :-) ."
I went to SSRL (Stanford) with a colleague who wanted to use liquid
propane. We had to go through a mound of paper work to get permission
bring propane on site and set up the experiments. I don't blame SSRL
for their safety policy, but I can clearly understand why liquid propane
is not commonly used.
If you don't think it is much of a danger, you might enjoy:
http://www.stupidvideos.com/video/stunts/propane_tank/#2974
You might also enjoy:
http://www.stupidvideos.com/video/stunts/C4_Propane_Explosion/#175408
Note: We did not bring any C-4 to SSRL :)
Steve
On 2/7/2012 10:50 AM, Bosch, Juergen wrote:
Hi Dirk,
I remember a neat paper don't recall who wrote it. I think it was in
Acta D where the authors made a tiny probe the size of an elongated
crystal glued to a [/Advertisement on] Hampton loop [/Advertisement
off]. The probe was a temperature sensor and they recorded the cooling
rate under different methods. The winner as far as I recall was
freezing in liquid propane for the lack of the missing gas layer, but
the second best method was LN2. Propane for whatever reason has gone
extinct in certain areas of the world :-) . I'll try to find that
reference but perhaps somebody else on this highly educated board
knows which paper I'm referring to. I want to say it was published
around 2004-2006.
Jürgen
On Feb 7, 2012, at 11:12 AM, Dirk Kostrewa wrote:
Dear Jürgen,
Am 07.02.12 16:58, schrieb Bosch, Juergen:
<snip>
Then one last remark, LN2 versus cryo-stream freeze. Dipping in LN2
leads to a quicker freeze of your material.
</snip>
Are you sure? There was a publication by Warkentin et al. [1] about a
cold gas layer above liquid nitrogen that reduces the expected cooling
rate a lot!
My very personal experience is, that cryo-cooling in the N2-stream
worked better for me than in LN2 in a variety of projects - but the
reason could just be me ;-)
Best regards,
Dirk.
[1] Matthew Warkentin, Viatcheslav Berejnov, Naji S Husseini, and Robert
E Thorne: "Hyperquenching for protein cryocrystallography", J. Appl.
Crystallogr., 39, 805-811 (2006)
--
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Dirk Kostrewa
Gene Center Munich
Department of Biochemistry
Ludwig-Maximilians-Universität München
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Germany
Phone: +49-89-2180-76845
Fax: +49-89-2180-76999
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WWW:www.genzentrum.lmu.de
*******************************************************
......................
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Office: +1-410-614-4742
Lab: +1-410-614-4894
Fax: +1-410-955-2926
http://web.mac.com/bosch_lab/
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