: [ccp4bb] Fwd: undefined edensity blob at glutamine
sidechain
dear all:
I. i am not able to fit trp,
since
1. trp doesnt fit well
2. positive density comes in fo-fc map after refinement
3. this is a soaked crystal str with heavy atom soln, the native one has
perfect density for gln, so mutation to trp
Well the density strongly suggests a Tryptophan side chain.
Sometimes discrepancies (errors) appear in sequences. How certain are
you of the accuracy of your sequence?
HTH,
Fred.
On 10/12/13 13:44, PriyankMaindola wrote:
dear members
i am trying to solve this crystal structure but
I am
check an anomalous map!
The obvious thing to do to rule out gold binding
-
Dr. Matthias Zebisch
Division of Structural Biology,
Wellcome Trust Centre for Human Genetics,
University of Oxford,
Roosevelt Drive,
Oxford OX3 7BN, UK
Phone (+44) 1865 287549;
Gesendet: Dienstag, 10. Dezember 2013 14:21
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re: [ccp4bb] Fwd: undefined edensity blob at glutamine sidechain
check an anomalous map!
The obvious thing to do to rule out gold binding
-
Dr. Matthias Zebisch
Division
It doesn't look like you left a CN on your gold atom. These things
are pretty much covalently bound.
Dale Tronrud
On 12/10/2013 08:13 AM, PriyankMaindola wrote:
dear all:
I. i am not able to fit trp,
since
1. trp doesnt fit well
2. positive density comes in fo-fc map after refinement
Priyank,
I think it's too far out from the Calpha to be Trp, and also not quite
flat enough. The map contains more information than just the shape as
to identity. Compare the max peak height of the 2Fo-Fc blob near the
Gln to neighboring element heights.
Much higher than C/N/O ? Probably
