[ccp4bb] How to improve the Se-Met crystal diffracted to 5.6A

2009-09-03 Thread xu zhen
Dear all, I have a Se-Met crystal diffracted to 5.6A and a full data set of the same native crystal diffracted to 2.8A. Since I can hardly improve the quality of the Se-Met crystal, may I ask some suggestion from the community about am I able to solve the structure based on the above

Re: [ccp4bb] How to improve the Se-Met crystal diffracted to 5.6A

2009-09-03 Thread Tim Gruene
Dear Zhen, you could try Santosh Panjikar's Autorickshaw server at http://www.embl-hamburg.de/Auto-Rickshaw/ to see if it finds a solution. Even risking to suggest something you already did or cannot carry out for some reason: Have you tried micro-seeding of the Se-Met drops with the

Re: [ccp4bb] How to improve the Se-Met crystal diffracted to 5.6A

2009-09-03 Thread artem
My suggestion would be to attempt finding Se atoms in the MAD dataset and then use these coordinates to do sulphur phasing - assuming of course that you can get the native crystals to diffract at an appropriately low-energy X-ray source. Artem Dear all, I have a Se-Met crystal diffracted to