P4BB@JISCMAIL.AC.UK
Betreff: [ccp4bb] AW: [ccp4bb] How to remove phospholipids bound to a
cytoplasmic protein
Dear Lionel,
In case of a lipid-binding protein, we were able to remove most (>90%) of the
bound lipids with a lipidex column. This could be verified by DSF since the
melting tempera
and.
Best,
Herman
-Ursprüngliche Nachricht-
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Lionel
Gesendet: Donnerstag, 26. Juni 2014 18:16
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [ccp4bb] How to remove phospholipids bound to a cytoplasmic protein
Dear all,
I wo
Hi Lionel,
A few musings/suggestions
If they are bound *inside* the protein, this suggests that the
phospholipids might be very tightly bound.
Do you have an affinity tag on your protein (e.g. His tag)? Perhaps you
might immobilise the protein on a suitable resin and wash with copious
amounts of
Dear all,
I would like to remove two phospholipids bound to (actually into) a cytoplasmic
protein. The protein was expressed in E. coli and the crystal structure
revealed the presence of two co-purified phospholipids (most probably DPPE).
A web search gave me three methods to remove bound lipi
