Re: [ccp4bb] Lysis of E coli

2014-01-28 Thread Roger Rowlett
We do not have experience with this product. We use a BeadBeater. Can 
handle up to 25-30 g of wet packed cells in the medium beater jar. The 
large jar will handle maybe 3-5x that, but I've never had to go to that 
scale.


___
Roger Rowlett
Gordon & Dorothy Kline Professor
Department of Chemistry
Colgate University
13 Oak Drive
Hamilton, NY 13346

tel: (315)-228-7245
ofc: (315)-228-7395
fax: (315)-228-7935
email: rrowl...@colgate.edu
On 01/28/2014 08:21 AM, Mark J van Raaij wrote:

Dear All,

Does anyone have experience with the FreezerMill for lysing E coli?
see:
http://www.spexsampleprep.com/products_by_category.aspx?cat=2
It seems to be more for "tissues", but perhaps it could also be used for lysing 
reasnoble quantities of E coli - the reason for asking is that I have to decide whether 
it would be useful for us and if so, to support its purchase.

Greetings,

Mark

Mark J van Raaij
Lab 20B
Dpto de Estructura de Macromoleculas
Centro Nacional de Biotecnologia - CSIC
c/Darwin 3
E-28049 Madrid, Spain
tel. (+34) 91 585 4616
http://www.cnb.csic.es/~mjvanraaij




[ccp4bb] Lysis of E coli

2014-01-28 Thread Mark J van Raaij
Dear All,

Does anyone have experience with the FreezerMill for lysing E coli?
see:
http://www.spexsampleprep.com/products_by_category.aspx?cat=2
It seems to be more for "tissues", but perhaps it could also be used for lysing 
reasnoble quantities of E coli - the reason for asking is that I have to decide 
whether it would be useful for us and if so, to support its purchase.

Greetings,

Mark

Mark J van Raaij
Lab 20B
Dpto de Estructura de Macromoleculas
Centro Nacional de Biotecnologia - CSIC
c/Darwin 3
E-28049 Madrid, Spain
tel. (+34) 91 585 4616
http://www.cnb.csic.es/~mjvanraaij