Re: [ccp4bb] Most Abundant Eukaryotic Membrane Protein List?

[David Waterman]

I wouldn't be so sure that HeLa is just XX, or that it should be called
garden variety after what I read today.
http://www.nature.com/news/most-popular-human-cell-in-science-gets-sequenced-1.12609#/b1

-- David


On 18 March 2013 15:08, Jacob Keller  wrote:

> Dear List,
>
> Does anyone know of a source of quantification of membrane proteins in
> garden-variety eukaryotic cell lines, e.g. HEK or HeLa cells (incidentally,
> it just occurred to me that probably all HeLa cells are XX--seems right,
> no?) I am looking for the highest-expressed, particularly, and definitely
> want to include single-pass proteins as well.
>
> Jacob
>
> --
> ***
>
> Jacob Pearson Keller, PhD
>
> Looger Lab/HHMI Janelia Farms Research Campus
>
> 19700 Helix Dr, Ashburn, VA 20147
>
> email: kell...@janelia.hhmi.org
>
> ***
>

Re: [ccp4bb] Most Abundant Eukaryotic Membrane Protein List?

[Soisson, Stephen M]

BLASPHEMY!  haha

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jacob 
Keller
Sent: Monday, March 18, 2013 1:22 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Most Abundant Eukaryotic Membrane Protein List?

I don't want to crystallize the protein--I have another reason

Jacob
On Mon, Mar 18, 2013 at 1:18 PM, Edward A. Berry 
mailto:ber...@upstate.edu>> wrote:
In the mitocondrial inner membrane (which is very protein-dense) I believe the 
most abundant protein is the adenine nucleotide transporter. (e.g. pdb1OKC).  
Single chain or homodimer, but apparently its not very easy to crystallize.


Jacob Keller wrote:
Dear List,

Does anyone know of a source of quantification of membrane proteins in 
garden-variety eukaryotic cell lines, e.g. HEK or
HeLa cells (incidentally, it just occurred to me that probably all HeLa cells 
are XX--seems right, no?) I am looking for
the highest-expressed, particularly, and definitely want to include single-pass 
proteins as well.

Jacob

--
***

Jacob Pearson Keller, PhD

Looger Lab/HHMI Janelia Farms Research Campus

19700 Helix Dr, Ashburn, VA 20147
email: kell...@janelia.hhmi.org<mailto:kell...@janelia.hhmi.org> 
<mailto:kell...@janelia.hhmi.org<mailto:kell...@janelia.hhmi.org>>

***




--
***

Jacob Pearson Keller, PhD

Looger Lab/HHMI Janelia Farms Research Campus

19700 Helix Dr, Ashburn, VA 20147

email: kell...@janelia.hhmi.org<mailto:kell...@janelia.hhmi.org>

***
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Re: [ccp4bb] Most Abundant Eukaryotic Membrane Protein List?

[Jacob Keller]

I don't want to crystallize the protein--I have another reason

Jacob

On Mon, Mar 18, 2013 at 1:18 PM, Edward A. Berry  wrote:

> In the mitocondrial inner membrane (which is very protein-dense) I believe
> the most abundant protein is the adenine nucleotide transporter. (e.g.
> pdb1OKC).  Single chain or homodimer, but apparently its not very easy to
> crystallize.
>
>
> Jacob Keller wrote:
>
>> Dear List,
>>
>> Does anyone know of a source of quantification of membrane proteins in
>> garden-variety eukaryotic cell lines, e.g. HEK or
>> HeLa cells (incidentally, it just occurred to me that probably all HeLa
>> cells are XX--seems right, no?) I am looking for
>> the highest-expressed, particularly, and definitely want to include
>> single-pass proteins as well.
>>
>> Jacob
>>
>> --
>> *
>>
>> Jacob Pearson Keller, PhD
>>
>> Looger Lab/HHMI Janelia Farms Research Campus
>>
>> 19700 Helix Dr, Ashburn, VA 20147
>>
>> email: kell...@janelia.hhmi.org 
>> > >
>>
>> *
>>
>
>


-- 
***

Jacob Pearson Keller, PhD

Looger Lab/HHMI Janelia Farms Research Campus

19700 Helix Dr, Ashburn, VA 20147

email: kell...@janelia.hhmi.org

***

Re: [ccp4bb] Most Abundant Eukaryotic Membrane Protein List?

[Edward A. Berry]

In the mitocondrial inner membrane (which is very protein-dense) I believe the most abundant protein is the adenine 
nucleotide transporter. (e.g. pdb1OKC).  Single chain or homodimer, but apparently its not very easy to crystallize.



Jacob Keller wrote:

Dear List,

Does anyone know of a source of quantification of membrane proteins in 
garden-variety eukaryotic cell lines, e.g. HEK or
HeLa cells (incidentally, it just occurred to me that probably all HeLa cells 
are XX--seems right, no?) I am looking for
the highest-expressed, particularly, and definitely want to include single-pass 
proteins as well.

Jacob

--
***

Jacob Pearson Keller, PhD

Looger Lab/HHMI Janelia Farms Research Campus

19700 Helix Dr, Ashburn, VA 20147

email: kell...@janelia.hhmi.org 

***

[ccp4bb] Most Abundant Eukaryotic Membrane Protein List?

[Jacob Keller]

Dear List,

Does anyone know of a source of quantification of membrane proteins in
garden-variety eukaryotic cell lines, e.g. HEK or HeLa cells (incidentally,
it just occurred to me that probably all HeLa cells are XX--seems right,
no?) I am looking for the highest-expressed, particularly, and definitely
want to include single-pass proteins as well.

Jacob

-- 
***

Jacob Pearson Keller, PhD

Looger Lab/HHMI Janelia Farms Research Campus

19700 Helix Dr, Ashburn, VA 20147

email: kell...@janelia.hhmi.org

***