Hello Chandra,
Soaking heavy atoms into crystals has a long and successful history (I use it
pretty often and it works much of the time). If you need phase information and
you have some crystals, I would certainly give it a try. There are many papers
and books to give you methods to do this
Dear Chandra,
A Million ways to die in the West (or when screening for HA derivatives).
But this has always worked for me:
https://www.cell.com/structure/fulltext/S0969-2126(00)00168-4
Just analyse ability to still form complex after modification.
Cheers,
Jesko
Am Mi., 2. Jan. 2019, 20:42 hat
Off the top of my head, assuming nothing much about your protein(s) - some
of this is only going to work if you can modify the protein(s) prior to
crystallization. This excludes the 'normal' heavy atom soaking type stuff
which can give rise to binding with covalent-like strength (while still
being
Dear CCP4 members, Can someone suggest to me a methodology to covalently modify
(non-cysteine) on component of a multicomponent complex with heavy atoms in
order to achieve better phase determination?
Thanks in advance
Chandra
kattamuricha...@yahoo.com
