Dear All,
I am looking for a bit of advice, and am interested to hear about
experiences people have had with various commercially-available 96-
well plate-based PCR, plasmid, and protein purification appliances and
plates. Forgive me for comparing commercial vendors.
We have a 96-well plate-compatible vacuum manifold (Qiavac). However,
the plasmid-purification kits from this vendor do seem comparatively
pricey to me. So, I am looking for a more cost-efficient system. How
compatible are 96-well PCR purification plates/plasmid-binding and
filter plates from different vendors with this type of vacuum
manifold? From what I can see, it becomes difficult to avoid cross-
contamination between wells, when using e.g. Whatman-type 96-well
microplates with Qiavac, because the distance between the drip
director of the DNA-binding/filter plate and a receiver plate (e.g.
PCR plate) in the manifold is relatively long, and the alignment isn't
great (drops end up on the rim). The alternative would be
centrifugation directly on top of a receiver plate, but I am concerned
about cross-contamination.
Which type of membranes/filter plates were people most pleased with
for (bacterial) lysate-clearing, avoiding clogging with lysates from ~
5 ml bacterial cultures; and which DNA/plasmid-binding plates have
most binding capacity and result in highest purity? Again cost-
efficient vendors would be preferred.
Regards,
Florian
