[ccp4bb] Off Topic- Fe-S electron transfer
Dear Community, I apologize for the off topic question but it is hard to resist since so many of our colleagues are incredibly knowledgeable. I am looking for a way to block or even remove the 2Fe-2S cluster in my enzyme. This cluster is involved in transferring electron from the Alpha to the Beta subunit and I wish to block this for an experiment. Does anyone know of a reagent or procedure that is known to do this (elegance is optional)? I first thought of EDTA but I don't think it will work given the covalent character of the Fe-S bonds. Thank you
Re: [ccp4bb] Off Topic- Fe-S electron transfer
Maybe there is a reason you haven't tried this, but can you just mutate the cysteines that ligate the cluster? Serine would be a nice choice. Mike - Original Message - From: Yuri Pompeu yuri.pom...@ufl.edu To: CCP4BB@JISCMAIL.AC.UK Sent: Tuesday, October 1, 2013 11:33:50 AM GMT -08:00 US/Canada Pacific Subject: [ccp4bb] Off Topic- Fe-S electron transfer Dear Community, I apologize for the off topic question but it is hard to resist since so many of our colleagues are incredibly knowledgeable. I am looking for a way to block or even remove the 2Fe-2S cluster in my enzyme. This cluster is involved in transferring electron from the Alpha to the Beta subunit and I wish to block this for an experiment. Does anyone know of a reagent or procedure that is known to do this (elegance is optional)? I first thought of EDTA but I don't think it will work given the covalent character of the Fe-S bonds. Thank you -- Michael C. Thompson Graduate Student Biochemistry Molecular Biology Division Department of Chemistry Biochemistry University of California, Los Angeles mi...@chem.ucla.edu
Re: [ccp4bb] Off Topic- Fe-S electron transfer
BAL (British anti-lewisite?) is supposed to destroy the FeS2 Rieske cluster in complex III Don't know how general it is. Yuri Pompeu wrote: Dear Community, I apologize for the off topic question but it is hard to resist since so many of our colleagues are incredibly knowledgeable. I am looking for a way to block or even remove the 2Fe-2S cluster in my enzyme. This cluster is involved in transferring electron from the Alpha to the Beta subunit and I wish to block this for an experiment. Does anyone know of a reagent or procedure that is known to do this (elegance is optional)? I first thought of EDTA but I don't think it will work given the covalent character of the Fe-S bonds. Thank you
