We have been using Strep-Tactin SuperFlow (high capacity) columns more than 100
times, even when run under preparative conditions with protein extracts from E.
coli fermentations.
Biotin binding to the engineered streptavidin is not irreversible but can be
cured by extended washing. To check
A couple of people asked why the GST/His steps. The only way I can expressed
this protein is by co-expression. Co-protein is GST tagged and my protein of
interest is N-term His and C-term StrepTag. Even with the coexpression, there
is a major degradation product, the reason why for the strep
Does anyone know how many times roughly you can re-use a Streptactin column? I
know that contamination with biotin will destroy the column but the protein
that I am using has gone through both a GST and Nickel purification steps
before seeing the Streptactin column and I think lately that I
