Thank you all for sharing your experience with Perfluoropolyether and freezing
membrane proteins !
Now I myself heard about one membrane protein where Perfluoropolyether was the
solution for the freezing problem. And it seems it worked in my case as well
:-)
Ulrike
Perhaps of interest here could be Santovac 5. It's an oil that does not
mix with anything, not water, not mineral oil, not alkanes, not
perfluoropolyether, not silicone oil, not paratone. Nothing but
acetone. It is a a polyphenyl ether that was originally developed as a
diffusion pump oil
Dear crystallographers,
does anyone have experience using Perfluoropolyether (Hampton Research) as
cryoprotectant for membrane proteins ?
Cheers,
Ulrike
Ulrike,
I usually suggest it as the second try (the first try is mother liquor
alone), as it does not involve mixing any new buffer concoctions. I do
not have hard data, but I'd estimate it worked in about 50% of cases; it
seemed not to matter if you try it on a soluble or membrane protein
