Re: [ccp4bb] Plate crystals

2012-10-16 Thread Tim Gruene
-BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Dear Jahan, is your diffraction useless, or do you call 4A resolution useless? 4A is not too bad and can be a start for structure determination while you are waiting for better crystals. You give very little information about what you actually have

[ccp4bb] Plate crystals

2012-10-16 Thread Patrick Shaw Stewart
Jahan It sounds as though the protein crystallizes well, so microseeding (done the right way) is very likely to solve the problem. Make a strong seed stock with as much crystalline material as possible from several wells, including different hits if possible. Just mix them all together, but

Re: [ccp4bb] Plate crystals

2012-10-16 Thread VAN RAAIJ , MARK JOHAN
apart from optimising the crystallisation conditions, it might be worth optimising the protein preparation or do some limited proteolysis - or even express short N-terminal and/or C-terminal deletions. Mark Quoting Patrick Shaw Stewart: Jahan It sounds as though the protein crystallizes

Re: [ccp4bb] Plate crystals

2012-10-16 Thread James Stroud
You might get better help if you post links (not attachments) to diffraction images from several angles and an image of the crystal that gave the image, and maybe several images of typical crystals. Also, you could post dimensions of the crystal, and if your lab is set up for it, a picture of

[ccp4bb] Plate crystals

2012-10-15 Thread Jahan Alikhajeh
Dear Friends, I am trying to crystalize a 70 kDa nasty protein but I got plate shape crystals with high mosaicity and useless diffraction (up to 4A). I tried to improve/optimize crystallization but either I got the same or nothing. I tried seeding but I had so many crystals without any

Re: [ccp4bb] Plate crystals

2012-10-15 Thread Parthasarathy, Gopal
] Plate crystals Dear Friends, I am trying to crystalize a 70 kDa nasty protein but I got plate shape crystals with high mosaicity and useless diffraction (up to 4A). I tried to improve/optimize crystallization but either I got the same or nothing. I tried seeding but I had so many crystals without

Re: [ccp4bb] Plate crystals

2012-10-15 Thread Raji Edayathumangalam
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jahan Alikhajeh [ja...@graduate.org] Sent: Monday, October 15, 2012 6:01 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Plate crystals Dear Friends, I am trying to crystalize a 70 kDa nasty

Re: [ccp4bb] Plate crystals

2012-10-15 Thread Sara Andres
tried Hampton's additive screen? Gopal From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jahan Alikhajeh [ja...@graduate.org] Sent: Monday, October 15, 2012 6:01 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Plate crystals Dear Friends