[ccp4bb] Spam : Re: [ccp4bb] Protein Ligand interaction using SPR technique

: [ccp4bb] Protein Ligand interaction using SPR technique Dear all, Sorry for off-topic question. I want to study protein interaction with few small molecules using SPR (Machine- *BIACORE 3000*). The recombinant protein expressed in bacterial expression system is of 92 kDa (with His tag), pI= 9

[ccp4bb] Spam : Re: [ccp4bb] Protein Ligand interaction using SPR technique

) Lab URL: http://www.cdfd.org.in/labpages/computational_functional_genomics.html - Original Message - From: Praveen Tripathi To: CCP4BB@JISCMAIL.AC.UK Sent: Fri, 31 Mar 2017 10:31:43 +0530 (IST) Subject: [ccp4bb] Protein Ligand interaction using SPR technique Dear all, Sorry for off

Re: [ccp4bb] Protein Ligand interaction using SPR technique

Dear Praveen, As said, Biacore3000 is not the best for small molecules, but in some case you can do nice things with this system. I see 3 possibilities : - You can immobilize your molecules on the chip if you have an amine on it which is supposed to not participate to the interaction. - You can

Re: [ccp4bb] Protein Ligand interaction using SPR technique

Hi Praveen, The BiaCore 3000 is an older model, and is not optimised for protein/small molecule interactions. The BiaCore T-100 and T-200 have enhanced sensitivity and are better suited small molecules (assuming you have to use SPR). As for chip choice, it depends an awful lot on the behaviour of

[ccp4bb] Protein Ligand interaction using SPR technique

Dear all, Sorry for off-topic question. I want to study protein interaction with few small molecules using SPR (Machine- *BIACORE 3000*). The recombinant protein expressed in bacterial expression system is of 92 kDa (with His tag), pI= 9. *Question-* 1. What should be the chip of choice- *NTA chi