Consider removing sulfate from the mother liquor after crystals are
formed. I recall having success replacing ammonium sulfate with sodium
malonate (it's a nice cryoprotectant as well). This is assuming that
ammonium sulfate is your major precipitant. If it's just an additive (say,
your
to this message and follow with
its deletion, so that we can ensure such a mistake does not occur in the future.
From: Goldman, Adrian
Sent: Sunday, February 17, 2019 10:48 PM
To: Keller, Jacob
Cc: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] SO4 or PO4
The total number of electrons in the two
ensure such a mistake does not occur in the future.
From: Eleanor Dodson
mailto:eleanor.dod...@york.ac.uk>>
Sent: Sunday, February 17, 2019 6:12 PM
To: Keller, Jacob mailto:kell...@janelia.hhmi.org>>
Cc: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: Re: [ccp4bb]
reply to this message and follow with
its deletion, so that we can ensure such a mistake does not occur in the future.
From: Eleanor Dodson
Sent: Sunday, February 17, 2019 6:12 PM
To: Keller, Jacob
Cc: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] SO4 or PO4
Well - I try to quantify the relative
Besides the anomalous difference peak which might be similar in height to
the anom diff peak from an intrinsic sulfur found in cysteine or
methionine, also consider hydrogen bonding.
In general, a sulfate will not be donating a hydrogen bond. A phosphate
probably will.
n consent of the sender.
> If you received this message by mistake, please reply to this message and
> follow with its deletion, so that we can ensure such a mistake does not
> occur in the future.
>
>
>
> *From:* CCP4 bulletin board *On Behalf Of *Yu Qiu
> *Sent:* Sunday, F
such a mistake does not occur in the future.
From: CCP4 bulletin board On Behalf Of Yu Qiu
Sent: Sunday, February 17, 2019 9:11 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] SO4 or PO4
Hi Shijun,
I had a similar issue a while ago that a acetylated lysine binding site was
occupied by acetate from
[mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of ???
Sent: Sunday, February 17, 2019 8:57 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [EXTERNAL] Re: [ccp4bb] SO4 or PO4
Dear all
Thanks for all your suggestions. PO4 is from my Ligand PI3P, but I cannot see
the electron density of the PI3P tail, and the binding site
electron density in the other
places, but is small than this one, and the diffraction resolution is 1.6A.
Best Regards
Shijun
-Original Messages-
From:"Roger Rowlett"
Sent Time:2019-02-17 00:47:55 (Sunday)
To: CCP4BB@JISCMAIL.AC.UK
Cc:
Subject: Re: [ccp4bb] SO4 or PO4
likely to
> give a clear result. If you could get to the sulfur & phosphorus
> edges, then you could (in principle) answer this, but that’s a very
> hard experiment to accomplish.
>
>
>> Begin forwarded message:
>>
>> *From: *jlliu20022002 liu > <mailto:jlliu200
Anomalous is the way to go but it helps to think about the final
concentrations used. With 50 mM PO4 (buffer) and 2 M SO4 (ammonium
sulfate) most "bindin sites in quesiton" will be occupied by SO4...
My 2 cents
Jeroen
On Sat, Feb 16, 2019 at 4:07 AM 张士军 <21620150150...@stu.xmu.edu.cn
ment to accomplish.
> Begin forwarded message:
>
> From: jlliu20022002 liu
> Subject: Re: [ccp4bb] SO4 or PO4
> Date: February 16, 2019 at 11:14:07 AM EST
> To: CCP4BB@JISCMAIL.AC.UK
> Reply-To: jlliu20022002 liu
>
> How about collect data at sulfur peak. You might s
Two things to look at that could provide a clue:
Examine the anomalous map for some density over the central atom. Sulfur
will often, but not always have significant anomalous density depending on
the wavelength and quality of data set.
Phosphate is normally HPO4= or H2PO4-. Look for phosphate
How about collect data at sulfur peak. You might see anomalous peak for
sulfur.
Jinyu
On Sat, Feb 16, 2019 at 4:07 AM 张士军 <21620150150...@stu.xmu.edu.cn> wrote:
> Dear all
>
> I have got a crystal grown at the condition both have ion of SO4 and PO4,
> and the diffraction resolution is very
Dear all
I have got a crystal grown at the condition both have ion of SO4 and PO4, and
the diffraction resolution is very well, but the problem is coming: how to tell
which is which just from electron density? I think they are exactly same.
Thanks a lot !!!
Beat Regards
Shijun
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