[0cab66323371-dmarc-requ...@jiscmail.ac.uk]
Sent: Tuesday, June 27, 2017 7:22 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Separating Monomers and Dimers
Dear all,
I am working on a Red Fluorescent Protein (His-Tag) molecular weight around
27kDa. After purification I ran a SDS page, the band at 27kDa
ubject: [ccp4bb] Separating Monomers and Dimers
Dear all,I am working on a Red FluorescentProtein (His-Tag) molecular weight
around 27kDa. After purification I ran a SDSpage, the band at 27kDa confirms
the monomer. The protein was stored at -20C, aweek later again I ran a gel,
this time I saw anoth
Hello Jaimohan,
Is it SDS-PAGE gel or Native PAGE gel ?. Theoretically SDS-PAGE is
supposed to show monomers and Native PAGE oligomers
On Tue, Jun 27, 2017 at 8:27 AM jai mohan <
0cab66323371-dmarc-requ...@jiscmail.ac.uk> wrote:
> Dear all,
> I am working on a Red Fluorescent
, 2017 8:41 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Separating Monomers and Dimers
it was not stable for frozen storage. if necessary，using protein fresh without
frozen
发自网易邮箱大师
在2017年06月27日 20:22，jai
mohan<mailto:0cab66323371-dmarc-requ...@jiscmail.ac.uk> 写道：
Dear all,
I am w
it was not stable for frozen storage. if necessary，using protein fresh without
frozen
发自网易邮箱大师
在2017年06月27日 20:22，jai mohan 写道：
Dear all,
I am working on a Red Fluorescent Protein (His-Tag) molecular weight around
27kDa. After purification I ran a SDS page, the band at 27kDa confirms the
Dear all,I am working on a Red FluorescentProtein (His-Tag) molecular weight
around 27kDa. After purification I ran a SDSpage, the band at 27kDa confirms
the monomer. The protein was stored at -20C, aweek later again I ran a gel,
this time I saw another new band between 50-60kDa, it confirmsthe
