[ccp4bb] Soaking at pH 4.0 ?

2010-03-12 Thread Paul Lindblom
Hi, I am trying to soak some sugars in my crystals, but the cystallization condition has a pH of 4.0. Does anybody has any experience with acidic oxidation in such a case. I think I can´t avoid oxidation at this pH? Any suggestions, or do I have to screen for other conditions? Thanks, P.

Re: [ccp4bb] Soaking at pH 4.0 ?

2010-03-12 Thread Nathaniel Clark
Hi, I have soaked various crystals at that pH with various sugars and never had a problem with oxidation of the sugar. I wouldn't worry about it. What group do you think will be oxidized? Nat On Fri, Mar 12, 2010 at 5:35 AM, Paul Lindblom lindblom.p...@googlemail.com wrote: Hi, I am trying

Re: [ccp4bb] Soaking at pH 4.0 ?

2010-03-12 Thread Maia Cherney
Could you transfer your crystals in a higher pH buffer? Maia Paul Lindblom wrote: Hi, I am trying to soak some sugars in my crystals, but the cystallization condition has a pH of 4.0. Does anybody has any experience with acidic oxidation in such a case. I think I can´t avoid oxidation at