PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Strange crystal packing in twined crystal -follow up
Dear all,
A couple of months ago I posted a message about strange crystal packing to the
bb. Thank you all for your insightful responses. When we were unable to find
any spurious electron
Dear all,
A couple of months ago I posted a message about strange crystal packing to the
bb. Thank you all for your insightful responses. When we were unable to find
any spurious electron density in the gap, we wondered if perhaps the twinning
caused us to impose too high symmetry on our data
Dear Florian,
I had a similar case for the membrane protein OmpF where the protein
stacked in columns, linked together by detergent belts acting as glue.
This allowed for 2 disctinct monoclinic space groups, one with tNCS and
the other one without. The detergent allowed for a rotation of a
Hmmm - is there a smaller lattice which might fit the density? As Andrew
says there are examples of pseudo emptiness in crystals but there are more
examples of wrong lattices!
Check for non-crystallographic translation?
You could attach the pointless/aimless/etc log files..
Eleanor
On Fri, 22 Jul
Dear Florian,
There have been previous examples where there are distinct
“layers” of protein packing without any obvious density linking the layers, as
it appears in the image you show. One is tyrosyl tRNA synthetase, where the
layers were in fact linked by a disordered
