Dear Rob,
at 1.5A resolution, you can comfortably run shelxe. If there is some
useful phase information in your solution, this should complete
your model, remove model bias and the resulting composition might tell
you the proper space group. Rename your PDB-file into 'mymodel.pda'
(pda, not pdb),
Hi Rob,
There is no information provided to think what might be the problem with
indexing. How confident are you in the direct beam coordinates?
Are any of the cell lengths long-ish?
It may not be enough but at a minimum, two images 90 degrees apart might be
helpful.
Preferably, they would be
Hello, yes, the difference map looks great. If this one diffracts to
near-atomic resolution, maybe you have a tetramer with a very low solvent
content. Cheers, Jon.C..
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Original Message
On 5 Jul 2021, 14:54, Robert S Phillips wrote:
> I collected
Why you have this problem I cannot guess, but your self-rotation clearly
shows 2 2 2 symmetry.
Try another data processing system?
Eleanor
On Mon, 5 Jul 2021 at 14:54, Robert S Phillips wrote:
> I collected data last week on crystals of tyrosine phenol-lyase obtained
> under new conditions.
