Nian,
Have you considered pressure-freezing, the method developed by Sol
Gruner's group at Cornell? If cryoprotectants are causing the problem,
freezing under pressure reduces the need for them. It's also possible
that your ligand is present but disordered in the crystal, and
Dear All,
I have been trying to get a protein-ligand complex crystal for a long
time. Here, ligand is either substrate or product for this kinase. I
have tried many methods: soaking with apo-crystal, co-crystal with
different concentration of ligand and screen for new conditions. I got
a couple
