If your signal goes all the way to the half Nyquist frequency, oversample your
map to have the pixel size equal to 1/3rd of the highest resolution. Your map
will look much better in coot. Real space fitting will work much better as
well.
Best,
Petr
> On May 17, 2017, at 11:24 AM, MyeongSeon
Hi,
CCP-EM (CCP4 sister project for cryoEM) can help you here. We have a EM
specific interface for several CCP4 programs as well as some others and
will handle map to mtz conversions for you.
You can download the suite here:
http://www.ccpem.ac.uk/download.php
We recently ran a workshop on high
On 17/05/2017 17:24, MyeongSeon Lee wrote:
Hi, all.
I have collected cryoEM data and want to use Coot and CCP4 program to build
model and to refine it.
1. What is the steps to do this?
2. How do I convert cryoEM map file to MTZ file?
CCPEM provides an increasingly sophisticated tool-set aim
Hi,
2. How do I convert cryoEM map file to MTZ file?
>
While technically you can do it, normally there is absolutely no need to do
it. In cryo-EM the map is your data, not reflection data (structure
factors!). So no need to 'massage' your data (the map) by converting it
into "Fobs" and storing as
Hi, all.
I have collected cryoEM data and want to use Coot and CCP4 program to build
model and to refine it.
1. What is the steps to do this?
2. How do I convert cryoEM map file to MTZ file?
3. Can I also use Phenix for this purpose?
Thanks to all for your help in advance.
