Ji,
probably the oil doesn't work and the glycerol gets you into problems
with phase separation in 2.5 M AmS
I recommend sucrose or other sugars with AmS - 20-22% (w/vol) should
do and you get no phase separation
At the same time you may need to increase the AmS considerably - often
the
You could use salts such as LiSO4 for cryo-protection (also amm sulphate
with small crystals has worked to a degree with us, when nothing else
worked, also 1.6-1.7 M amm. sulphate was exchangable to 40% PEG 400 (very
quick), although with not so great results...).
which oil did you try? that
Hi Ji,
Our lab has had good luck using sodium malonate to cryoprotect salt-
grown crystals. See:
Acta Cryst. (2003). D59, 2356-2358
Malonate: a versatile cryoprotectant and stabilizing solution for salt-
grown macromolecular crystals
T. Holyoak, T. D. Fenn, M. A. Wilson, A. G. Moulin, D.
Dear,
I have a crystal diffracted anisotrophically. I tested with a few
different cryo conditions like oil, glycerol in different
concentration to get a better data but these conditions didn't help
any.
Using capillary method improved the diffraction (isotrophic) but the
crystal couldn't survive
Look at the paper (search in pubmed) by B. M. Schick and F. Jurnak on
cryoprotection of TuTs. He changes the reservoir in 24 steps using a 24
well cryschem plate. There are some references there that are important
too.
Jackie Vitali
On Mon, Jun 23, 2008 at 8:14 PM, Ji lee [EMAIL PROTECTED]
