Re: [ccp4bb] commercially available proteins

2012-04-27 Thread Stephen Weeks
Just in case you are blocking with BSA, how about, e. coli beta-galactosidase (114 KDa) ?. It's commercially available but you probably have cells already making it in the lab On 26 April 2012 19:29, Peter Hsu hsuu...@u.washington.edu wrote: Hi all, Sorry for the very off topic problem. I'm

[ccp4bb] commercially available proteins

2012-04-26 Thread Peter Hsu
Hi all, Sorry for the very off topic problem. I'm looking for a loading control for my westerns (doing in vitro assays). Due to separation/antibody specificity issues, I need super good separation between 70 and 100 kDa, oftentimes running those markers all the way to the bottom of the gel. If

Re: [ccp4bb] commercially available proteins

2012-04-26 Thread Bosch, Juergen
How about plain old BSA ? Jürgen On Apr 26, 2012, at 1:29 PM, Peter Hsu wrote: Hi all, Sorry for the very off topic problem. I'm looking for a loading control for my westerns (doing in vitro assays). Due to separation/antibody specificity issues, I need super good separation between 70 and