ODD INDEED!
When you get crystals with the same morphology for three different
proteins in same condition
you should start suspecting that they are not protein crystals but
something to do with
a combination of the common buffer for each of the proteins or/and the
precipitant used
Enrico.
If I remember correctly, NaF forms octahedral crystals. Be sure to
check for salt crystals in your reservoir well.
ho
All the crystals I got for three different proteins in same condition looked
similar. I think crystal morphology may vary with the crystallizing conditions.
Hi all,
I found some microcrystals the other day in some trays grown at 4C. Strangely
enough, they disappeared the next day after putting them back in the fridge
overnight, but after letting the tray sit at RT for 5-10 min, the microcrystals
reappeared. Leaving them at RT for 2-3 hrs, and then
I used NaF in the precipitant thrice for three different proteins to be
crystallized each time I got crystals and they were all salt crystals ! I
thought low solubility of NaF causes this quick crystallization of salt.
Recently I got crystals of a protein at 20 degree grown in gradient
FWIW it's still at
http://web.archive.org/web/20050124153157/http://microgravity.msfc.nasa.gov/snell/vibration.html
HTH,
Kay
David Briggs schrieb:
Hi all
I'm doing a quick talk on crystals/crystallography for a lay
audience in a couple of weeks time, and I'm looking for some time
lapse
Hi all
I'm doing a quick talk on crystals/crystallography for a lay
audience in a couple of weeks time, and I'm looking for some time
lapse movies / animated gifs of crystal growth.
The one I was thinking of using was here:
http://microgravity.msfc.nasa.gov/snell/vibration.html
But that link is
There is a good one of lysozyme crystal growth on Bernhard Rupp's website:
http://www.ruppweb.org/level1/movies_list.htm
http://www.ruppweb.org/level1/movies_list.htmThe Microcrystallization
movie taken with the Cryscam is the one I speak of.
On Wed, Jul 21, 2010 at 11:40 AM, David Briggs
Dear David,
You are welcome to use this movie from our homepage:
http://shelx.uni-ac.gwdg.de/xtal/xtal.htm#xtalgrowth
Best wishes, George
Prof. George M. Sheldrick FRS
Dept. Structural Chemistry,
University of Goettingen,
Tammannstr. 4,
D37077 Goettingen, Germany
Tel. +49-551-39-3021 or -3068
