try to change ur buffer instead of PBS u can use Tris or HEPES. In PBS
buffer u will get salt crystals in many condition becoz of
phopshaphate try to use nonionic detergents in ur soluton ..
On Fri, May 20, 2011 at 8:29 PM, Nian Huang wrote:
> It might be SDS precipitation. Al
It might be SDS precipitation. Although 0.1% SDS is generally considered not
high enough to precipitate at 4 degree, it might interact with other
components in your solution to form even less soluble material.
Nian
On Fri, May 20, 2011 at 9:36 AM, WEI MIN wrote:
> Dear All
>
> I have a difficu
Hi Wei,
this milk is precipitated/microcrystals of SDS probably with some phosphate
since you are in PBS buffer.
I would have two suggestions.
1-Can't you find a better detergent than SDS for your membrane protein? Have
you run a detergent screen for this protein to find a milder and more
crystalli
Tel: (630)252-0665
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From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
WEI MIN
Sent: Friday, May 20, 2011 9:36 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] crystallization of a weird protein
Dea
Dear All
I have a difficulty to crystallize my membrane protein(his tagged). I got
the salt crystals from many different screening conditions. The protein is
in PBS buffer and 130mM salt with 0.1% SDS. I store It in the 4 degree
although it forms the milk solution.
The solution is getting clear in
