gt; Jacob Keller
>>
>>
>> ***
>> Jacob Pearson Keller
>> Northwestern University
>> Medical Scientist Training Program
>> Dallos Laboratory
>> F. Searle 1-240
>> 2240 Campus Drive
>> Evans
***
>
> - Original Message -
> From: R.M. Garavito
> To: CCP4BB@JISCMAIL.AC.UK
> Sent: Tuesday, August 04, 2009 12:37 PM
> Subject: Re: [ccp4bb] detergent crystals
>
>
> Parveen,
>
>
> Bert and Pa
AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] detergent crystals
Hi All,
I got some hexagonal crystals in one of my crystal condition. The protein is
a membrane protein and contains 0.05% DDM. Has anybody seen DDM crysals and
if yes, how do they look like?
thanks in advance
Parveen Goyal
To: CCP4BB@JISCMAIL.AC.UK
Sent: Tuesday, August 04, 2009 12:37 PM
Subject: Re: [ccp4bb] detergent crystals
Parveen,
Bert and Pascal are correct in that most alkyl glycoside detergent are
notoriously difficult to crystallize in aqueous solution when you have the
beta-anomer (what we no
Parveen,
Bert and Pascal are correct in that most alkyl glycoside detergent are
notoriously difficult to crystallize in aqueous solution when you have
the beta-anomer (what we normally buy). However, the alpha-anomers
can be quite easy to crystallize and can contaminate batches of beta-
a
Hi Jose,
It is quite difficult to crystallize DDM. The main problem is to estimate
detergent concentration in the final sample.
One method requires the use of chromatographic workstation coupled to a
light scattering detector and refraction index measurement unit. In this
case you can know if there
tp://www.umassmed.edu/pmm/faculty/vandenberg.cfm
-Original Message-
From: CCP4 bulletin board on behalf of Jose Antonio Cuesta Seijo
Sent: Tue 8/4/2009 12:24 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] detergent crystals
Hi Parveen.
DDM crystals can look a bit like that. Like a pe
Hi Parveen.
DDM crystals can look a bit like that. Like a pencil that has been
sharpened from both ends until there is very little pencil left, but often
the ends are flatter than that. You can get tons of these (also with DM) in
conditions with medium weight PEGs. Of course many proteins will als
: [ccp4bb] detergent crystals
Hi All,
I got some hexagonal crystals in one of my crystal condition. The
protein is a membrane protein and contains 0.05% DDM. Has anybody seen
DDM crysals and if yes, how do they look like?
thanks in advance
Parveen Goyal
Hi All,
I got some hexagonal crystals in one of my crystal condition. The protein is
a membrane protein and contains 0.05% DDM. Has anybody seen DDM crysals and
if yes, how do they look like?
thanks in advance
Parveen Goyal
Jacob,
DDM should never crystallize under any aqueous conditions seen for
protein crystallization. We tend to store 20% stock solutions (~0.4
M) of DDM in buffer at 4C. DDM tends to phase out long before it
would crystallize (~ 0.8 mol percent).
With this said, how have you set up the s
The carry-over would be DDM, and my guess would be DDM crystals formed
upon dehydration. The DDM concentration will be huge after repeated
dilution/concentration cycles. DDM micelles will hardly pass the 50
kDa cut-off filter so imagine how much you have added!
Poul
On 31/10/2008, at 02.21,
Dear Crystallographers,
has anyone heard of or seen crystals form in their membrane protein stocks
in the presence of DDM, such as from a breakdown product, or otherwise? My
protein stock, after having been stored at ~2mg/mL (around solubility limit)
at 4degC in (theoretically) nothing but ~5m
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