Re: [ccp4bb] dissolving peptides !

[Frank Niesen]

Rashmi,
To facilitate dissolution of a peptide we found ultrasound (in glass, plastics 
absorb much of the energy) useful. It might be also helpful to warm the 
solution mildly.
Frank

[ccp4bb] dissolving peptides !

[rashmi panigrahi]

Hello everybody,
I have a 16 mer peptide which is predicted to be positively charged alpha
helix and has 50% of its sequence hydrophobic.

Could any one recommend the best way to dissolve it, and then it can be used
for crystallization trials.

thanks

-- 
rashmi

Re: [ccp4bb] dissolving peptides !

[gauri misra]

Rashmi,
You can try 1%DMSO and also acetonitrile but the former is preferable.

Gauri

On Mon, Jun 7, 2010 at 3:04 AM, rashmi panigrahi 
rashmi.panigrah...@gmail.com wrote:

 Hello everybody,
 I have a 16 mer peptide which is predicted to be positively charged alpha
 helix and has 50% of its sequence hydrophobic.

 Could any one recommend the best way to dissolve it, and then it can be
 used for crystallization trials.

 thanks

 --
 rashmi