Hi Pascal,
Can you give us some details about your experimental setup?
If I had to guess, are you using monochrometers set to approximately 2x Trp
EX/EM. You may be able to solve this by just adding some cheap colored glass
high pass filters to the optical path.
Shae Padrick
On Apr 23, 2015
I have not seen this with mbp before, however you could try fret instead...
Artem
On Apr 23, 2015 9:26 PM, "Pascal Egea" wrote:
> Dear All,
>
> This is more a biochemical/biophysics question but since we need to
> complete our structural analysis with functional or biophysical data, I
> thought
Dear All,
This is more a biochemical/biophysics question but since we need to
complete our structural analysis with functional or biophysical data, I
thought I would ask. We crystallized a protein as an MBP fusion and solved
its structure. Based on previous knowledge we know that the small protein
