Hi Jason I don't know what is your refinement protocol, but I said that at this resolution you can try to refine only two B factors per each aminoacid : one for the main chain and one for the side chain. There is an option to do that in CNS_SOLVE. You can find very nice examples in the CNS_SOLVE tutorial pages.
Hope it helps. Cheers and good luck ! Francisco > Hi All, > > I just refined a structure at 2.95A and getting a good R-work and > R-free(0.236 and 0.274). When I checked the PDB, I found many residues > have > high temperature factor over 100. How can I use Phenix or CNS fix those > high > temperature factor for further refinment? > > Any suggestions are welcome. Thank you > Jason > -- ----------------------------------------- Francisco J. Enguita, Ph.D. Host-pathogen Interactions Group Macromolecular Crystallography Laboratory ITQB EAN, Av. da República 2781-901 Oeiras Portugal Phone : +351-21-4469663 Fax : +351-21-4433644 E-mail : [EMAIL PROTECTED] -----------------------------------------
