Re: [ccp4bb] how to optimize crystal without sharp boundary

2009-05-20 Thread Kay Diederichs 

Hi Jerry,

did you check out the CCP4 wiki - there's a lot of information at 
http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Crystals


HTH,

Kay

Jerry McCully schrieb:

Dear All:

 Recently we got some crystals from  ammonium sulfate and PEG400. 
They look like hexagonal single crystals but without sharp boundary.   
They did not diffract well.


 This protein can also be crystallized from PEG6000 in star shape. 
We tried hampton additive screen but can not turn them into single crystals.


 Can anyone give some suggestions to optimize such crystals?


 Thanks a lot,

Jerry


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[ccp4bb] how to optimize crystal without sharp boundary

2009-05-20 Thread Jerry McCully 

Dear All:

 Recently we got some crystals from  ammonium sulfate and PEG400. They look 
like hexagonal single crystals but without sharp boundary.   They did not 
diffract well.

 This protein can also be crystallized from PEG6000 in star shape. We tried 
hampton additive screen but can not turn them into single crystals.


 Can anyone give some suggestions to optimize such crystals?


 Thanks a lot,

Jerry 

_
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