Re: [ccp4bb] large number in ASU

2017-04-27 Thread Eleanor Dodson
Have you looked at a self rotation function? Is there a clear 3-fold axis perhaps? Eleanor On 27 April 2017 at 10:45, Mark J van Raaij wrote: > Does the self-rotation function suggest presence of NCS axes? If so, this > may help you figure out the symmetry inside the

Re: [ccp4bb] large number in ASU

2017-04-27 Thread Andrew Leslie
Dear Jademilson, At a CCP4 APS workshop a few years ago, one of the students solved a molecular replacement problem where I think there were close to 40 copies in the asu. I have to say that many people were surprised by this, but I think the protein was smaller and

Re: [ccp4bb] large number in ASU

2017-04-26 Thread CRAIG A BINGMAN
Congratulations! I think you are now looking for additional crystallographic and non-crystallographic symmetry, because finding 40 particles in arbitrary positions and orientations is going to be brutal. I wouldn’t take the cell and point group assignment from XDS at face value. Rather I

Re: [ccp4bb] large number in ASU

2017-04-26 Thread Keller, Jacob
[mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jademilson Santos Sent: Wednesday, April 26, 2017 3:35 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] large number in ASU Greetings all, I am having trouble with a data set and would like to know if somebody can help. I'm working with a protein of approximately 50