Re: [ccp4bb] largest protein crystal ever grown?

2013-10-25 Thread Alexander Aleshin
מאת: simon.phill...@rc-harwell.ac.uk<mailto:simon.phill...@rc-harwell.ac.uk> תאריך: אל: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> נושא: Re: [ccp4bb] largest protein crystal ever grown? Hi Derek, That brings back memories. I am pretty certain that is the myoglobin crystal th

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-25 Thread R. M. Garavito
Cellular: 0547 459 608 >>> >>> On Oct 25, 2013, at 12:18 , Boaz Shaanan wrote: >>> >>>> Hi, Referring to the Hb crystal that Bill Scott saw in the MRC crystal >>>> growing room (by now "tho old one" I guess), is that the one that

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-25 Thread Felix Frolow
telling my students about it when they ask about crystal size limits. >>> Cheers, Boaz >>> >>> >>> >>> הודעה מקורית >>> מאת: simon.phill...@rc-harwell.ac.uk >>> תאריך: >>> אל: CCP4BB@JISCMAIL.AC.UK >>>

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-25 Thread Derek Logan
m: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Derek Logan Sent: 24 October 2013 19:08 To: ccp4bb Subject: Re: [ccp4bb] largest protein crystal ever grown? Hi, Last spring I visited the Protein Crystallography Station at Los Alamos. On a shelf, in a capillary in a serious exh

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-25 Thread Felix Frolow
ך: > אל: CCP4BB@JISCMAIL.AC.UK > נושא: Re: [ccp4bb] largest protein crystal ever grown? > > > Hi Derek, > > That brings back memories. I am pretty certain that is the myoglobin crystal > that was already on Benno's shelf at Brookhaven when I went there in 1980 to &g

[ccp4bb] השב: Re: [ccp4bb] largest protein crystal ever grown?

2013-10-25 Thread Boaz Shaanan
ystal size limits. Cheers, Boaz הודעה מקורית מאת: simon.phill...@rc-harwell.ac.uk תאריך: אל: CCP4BB@JISCMAIL.AC.UK נושא: Re: [ccp4bb] largest protein crystal ever grown? Hi Derek, That brings back memories.  I am pretty certain that is the myoglobin cr

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-25 Thread Simon . Phillips
(sec)    +44 (0)7884 436011 (mobile) www:   www.rc-harwell.ac.uk -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Derek Logan Sent: 24 October 2013 19:08 To: ccp4bb Subject: Re: [ccp4bb] largest protein crystal ever grown? Hi, Last spring I visite

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-24 Thread Tobias Beck
Dear all, dear John, Thanks for the replies and references! I like the ice cream tub setup. Best wishes, Tobias. On Thu, Oct 24, 2013 at 10:05 PM, Jrh wrote: > Dear Tobias, > There is also this one :- http://dx.doi.org/10.1107/S0021889801007245 > In this study we had to use a smaller crystal

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-24 Thread Jrh
Dear Tobias, There is also this one :- http://dx.doi.org/10.1107/S0021889801007245 In this study we had to use a smaller crystal than the largest ones available of 125mm3. They were a lovely rhombic dodecahedral crystal habit. Nb we only published details of the size of the one used. These crysta

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-24 Thread Derek Logan
Hi, Last spring I visited the Protein Crystallography Station at Los Alamos. On a shelf, in a capillary in a serious exhibition-quality glass dome, was a crystal of myoglobin some 50 mm**3, if I remember correctly. I was told it had been made by Benno Schoenborn some decades earlier and had bee

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-24 Thread Victor Lamzin
Also following on from John's comment - back to the times of my PhD I was repeatedly growing crystals of bacterial formate dehydrogenase (80 kDa) of a size about 7x1.5x1 mm. I thought that was quite normal and did not even think of making a photo of 'just a protein crystal'. Victor

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-24 Thread David Briggs
Following on from John's comment, when I did my PhD at Birkbeck in the early 2000s, one of David Moss's other PhD students (John Bond) grew some gigantic (>1cm edges) crystals of things like HEWL & Myoglobin, which he then (somewhat perversely) crushed to load into capillaries for powder diffractio

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-24 Thread Jrh
Dear Tobias, Take a look at http://dx.doi.org/10.1107/S0108767389012912 The ribonuclease crystal I used to measure the speed of sound, using laser generated ultrasound, was of volume 129 mm3 ie 7.7x6.2x2.7 mm . David Moss of Birkbeck College provided it. Best wishes, John Prof John R Helliwell

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-24 Thread Simon . Phillips
CP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Tobias Beck Sent: 24 October 2013 16:34 To: ccp4bb Subject: [ccp4bb] largest protein crystal ever grown? Dear all, I was just wondering if anyone has some information or references about the dimensions of the largest protein crystal ever grown? I

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-24 Thread William G. Scott
I remember seeing an approx (5mm)^3 haemoglobin crystal in the MRC LMB crystal growing room, and the nucleosome crystals there were almost as big in their longest dimension. Bill On Oct 24, 2013, at 8:33 AM, Tobias Beck wrote: > Dear all, > > I was just wondering if anyone has some informat

Re: [ccp4bb] largest protein crystal ever grown?

2013-10-24 Thread Frank von Delft
Very recently (last few months), 3x1x1mm monsters for neutrons. I think J. Crystal Growth. On 24/10/2013 16:33, Tobias Beck wrote: Dear all, I was just wondering if anyone has some information or references about the dimensions of the largest protein crystal ever grown? I am aware that for

[ccp4bb] largest protein crystal ever grown?

2013-10-24 Thread Tobias Beck
Dear all, I was just wondering if anyone has some information or references about the dimensions of the largest protein crystal ever grown? I am aware that for neutron protein crystallography one usually needs crystals with mm dimensions. I have found some information on crystallization under micr