Sebastiano,
I have had really nice success by mixing initial crystallization hits back in
to sparse matrix screen conditions. My personal favourite is using the Wizard
screens.
http://www.rigakureagents.com/p-1-wizard-classic-crystallization-screen-series.aspx
I found 75% of the
Hi Sebastian,
you're thinking about local sparse matrix screening. I've done this at a
90:10 ratio.
Majeed, S., Ofek, G., Belachew, A., Huang, C.C., Zhou, T., and Kwong, P.D.
Enhancing protein crystallization through precipitant synergy.
Structure. 2003; 11: 1061–1070
All best.
Andreas
On
Dear all,
I recall a paper (or was is a talk at a conference?) describing the
optimisation of initial hits of crystallisation by using a standard screen kit
as additive.
Something like setting the tray using the initial crystallisation hit condition
in the reservoir and mixing 75% of the hit
