[ccp4bb] low resolution of DNA binding protein

2012-01-06 Thread LISA
Hi all, I have a DNA binding protein. I get crystals of this protein by co-crystallization with different dsDNAs. But all the crystals have very poor resolution, about 10-20A. I tried to purify protein-DNA complex before setting trays, but it didn't work. Please give me some suggestion. Thanks.

Re: [ccp4bb] low resolution of DNA binding protein

2012-01-06 Thread Ed Pozharski
On Fri, 2012-01-06 at 20:40 +0800, LISA wrote: Hi all, I have a DNA binding protein. I get crystals of this protein by co-crystallization with different dsDNAs. But all the crystals have very poor resolution, about 10-20A. I tried to purify protein-DNA complex before setting trays, but it