Pascal,
The reason this phenomenon looks odd to you is that detergent phase separation
is not a micelle size phenomenon, but a micelle surface phenomenon. Like any
colloidal solution, even protein, there can be conditions where the colloidal
particles aggregate (the cloud point), creating a mi
Hi Pascal,
this behavior doesn't surprise me.
We had crystals in FC12, and didn't get any phase transitions in any
PEGs from 200 to 20.000, and with or w/o organic solvents such as MPD.
And I can tell you we had a lot of detergent with the purified protein,
concentrating on 50kDa.
With lipids,
this phase separation cluster
into certain precipitant groups or salts by any chance (ie, only low MW PEGs,
only in MPD, etc)?
Cheers,
Nick
From: Pascal Egea [mailto:pas...@msg.ucsf.edu]
Sent: Thursday, August 01, 2013 6:24 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] membrane protein and
Dear All,
I have a question tailored for the membrane protein and detergent folks. We
are purifying a membrane protein that associates into an homoligomeric pore
and we have been successfully preparing it in two detergents: FC-12 or a
mild lipid. The two Protein Detergent Complexes look very homog
