Re: [ccp4bb] off topic: Fluorescence labeled protein, neutral at pH=7
Hi Xiang. If you are doing in vitro experiments like binding studies then try BODIPY Fluorophores. It conjugates the protein. Advantage is molecular weight of these BODIPY Fluorophores is few hundreds Da. Also, these dyes have well separable absorption and emission spectrums. Good luck Raj On Mon, Sep 16, 2013 at 9:24 PM, 李翔 lixiang1...@gmail.com wrote: Hi everyone, I want to get some fluorescence labeled protein for my experiment. I wish it is under 40K Da and have isoelectric point of 7 or slightly lower. Do you have any suggestions on the commercially available protein please? Thanks very much for your help! Sincerely, Xiang -- Li Xiang Department of chemistry, Purdue University Email：lixiang1...@gmail.com
[ccp4bb] off topic: Fluorescence labeled protein, neutral at pH=7
Hi everyone, I want to get some fluorescence labeled protein for my experiment. I wish it is under 40K Da and have isoelectric point of 7 or slightly lower. Do you have any suggestions on the commercially available protein please? Thanks very much for your help! Sincerely, Xiang -- Li Xiang Department of chemistry, Purdue University Email：lixiang1...@gmail.com
Re: [ccp4bb] off topic: Fluorescence labeled protein, neutral at pH=7
Deal Xiang, You can think of GFP/YFP protein attached with any protein would be good for your experiment. These can be easily cloned and expressed. Alternatively one more approach is mentioned below. Chose a protein which has only one cysteine, that too at the surface then you can think of labelling your protein with thiol-ester of commerically available atto-dyes. You can also think of mutating alanine present at the surface with cysteine which subsequently can be covalently attached with the thio-reactinve dyes. It is two step process, first you have to set up a chemical reaction linking the thiol mediated covalent ligation of dyes to your protein, then further purifying the lablled protein with HPLC. On 17 September 2013 00:54, 李翔 lixiang1...@gmail.com wrote: Hi everyone, I want to get some fluorescence labeled protein for my experiment. I wish it is under 40K Da and have isoelectric point of 7 or slightly lower. Do you have any suggestions on the commercially available protein please? Thanks very much for your help! Sincerely, Xiang -- Li Xiang Department of chemistry, Purdue University Email：lixiang1...@gmail.com
