You should add some salt when you anneal!!!
The duplex is highly negatively charged, so adding even a
small amount (like 10mM NaCl) will help with charge
screening, thus making the two strands less repellant to
each other. Buffer is also always a good idea. At low pH
and high temp you'll hy
ne 22, 2008 1:01 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] query on DNA-protein complex preparation for
crystallization
Hi
Thank you for the mail.
It seems your correct. A(260 nm)/A(280) of one oligo
is around 1.0 and peak is around 272. Other
oligo's(260 nm)/A(280) is around 1.5.
Can I
does dissolve, what pH
> does
> >> it have? Does it run on
> >> an agarose gel? When you ignite a speck of it on
> a
> >> clean metal spatula -
> >> does it burn or does it just sit there (and what
> >> color does it become).
> >>
> >> Norm
E
rajakumar
Sent: Saturday, June 21, 2008 5:48 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] query on DNA-protein complex
preparation for
crystallization
Dear All
Sorry for non-crystallography question. I have
synthesized two complementary strands of 16 bases in
length for making duplex DNA and c
> -Original Message-
> From: CCP4 bulletin board
> [mailto:[EMAIL PROTECTED] On Behalf Of E
> rajakumar
> Sent: Saturday, June 21, 2008 5:48 PM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] query on DNA-protein complex
> preparation for
> crystallization
>
lletin board [mailto:[EMAIL PROTECTED] On Behalf Of E
rajakumar
Sent: Saturday, June 21, 2008 5:48 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] query on DNA-protein complex preparation for
crystallization
Dear All
Sorry for non-crystallography question. I have
synthesized two complementary strands o
Dear All
Sorry for non-crystallography question. I have
synthesized two complementary strands of 16 bases in
length for making duplex DNA and co-crystallization
with DNA binding protein. I have mixed two
complementary strands of 1:1 molar ratio (0.5 mM) in
water and concentrated to 1.5 mM (Duplex),
