Re: [ccp4bb] question about arginine sepharose 4B

2013-04-24 Thread Artem Evdokimov
Hi, You can make it :) There are a number of good ways to do so, but my favorite one is to order Cys-Arg dipeptide and then use iodoacetamide-agarose resin (very cheap) to load up the peptide. This method allows you to quantify the loading, if you care to know how much you immobilized and also

[ccp4bb] question about arginine sepharose 4B

2013-04-23 Thread Jacqueline Vitali
Dear all, Arginine sepharose 4B is an affinity column. If you have used it please let me know where you purchased it. GE Lifesciences used to sell it but discontinued it. I found it in China in the site http://www.pharmaceutical-sales.net and I corresponded with their sales representative