Dear All
just want to ask the difference between labelling your protein with D L
selenomethionine mixture and L selenomethionine alone..will it make
difference in anomalous diffraction,detection of Se signal and extraction
of phases. Or will be the same for both..?
--
Regards
Faisal
School of
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Dear ,
I am not up to date, but is there an expression system that allows you
to incorporate D-amino acids into proteins? In terms of phasing only
the Se-atom is a point or sphere, so the signal won't be altered
provided the position of that atom
Subject: Re: [ccp4bb] seleno methionine labelling of protein
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Dear ,
I am not up to date, but is there an expression system that allows you
to incorporate D-amino acids into proteins? In terms of phasing only
the Se-atom is a point or sphere, so
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From: Tim Gruene t...@shelx.uni-ac.gwdg.de
To: CCP4BB@JISCMAIL.AC.UK
Sent: Monday, November 12, 2012 4:25:34 AM
Subject: Re: [ccp4bb] seleno methionine labelling of protein
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Dear ,
I am not up to date, but is there an expression system