[ccp4bb] seleno methionine labelling of protein

2012-11-12 Thread Faisal Tarique
Dear All just want to ask the difference between labelling your protein with D L selenomethionine mixture and L selenomethionine alone..will it make difference in anomalous diffraction,detection of Se signal and extraction of phases. Or will be the same for both..? -- Regards Faisal School of

Re: [ccp4bb] seleno methionine labelling of protein

2012-11-12 Thread Tim Gruene
-BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Dear , I am not up to date, but is there an expression system that allows you to incorporate D-amino acids into proteins? In terms of phasing only the Se-atom is a point or sphere, so the signal won't be altered provided the position of that atom

Re: [ccp4bb] seleno methionine labelling of protein

2012-11-12 Thread Chittaranjan Das
Subject: Re: [ccp4bb] seleno methionine labelling of protein -BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Dear , I am not up to date, but is there an expression system that allows you to incorporate D-amino acids into proteins? In terms of phasing only the Se-atom is a point or sphere, so

Re: [ccp4bb] seleno methionine labelling of protein

2012-11-12 Thread Faisal Tarique
- From: Tim Gruene t...@shelx.uni-ac.gwdg.de To: CCP4BB@JISCMAIL.AC.UK Sent: Monday, November 12, 2012 4:25:34 AM Subject: Re: [ccp4bb] seleno methionine labelling of protein -BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Dear , I am not up to date, but is there an expression system