Thanks everybody for your replies. I am having another look at my data in
P1 and will post an update and summary to the list.
--
Kevin Jude, PhD
Structural Biology Research Specialist, Garcia Lab
Howard Hughes Medical Institute
Stanford University School of Medicine
Beckman B177, 279 Campus
incompatible with cell dimensions
EXTERNAL : Real sender is owner-ccp...@jiscmail.ac.uk
Hello community, I wonder if I could solicit advice about a problematic
dataset. I plan to solve the structure by molecular replacement and expect that
the protein is relatively compact, ie not elongated. SAXS data
Hmm - that sym op means you have a near C centred cell with spacegroup C 2
2 2 ?
Maybe some of your protein has been chewed up? That does happen?
How good is the diffraction?
Eleanor
On Sat, 1 Jun 2019 at 17:44, Jonathan Cooper <
0c2488af9525-dmarc-requ...@jiscmail.ac.uk> wrote:
> Does
Does the SAXS model contain more than one subunit? If so, I would be tempted to
go back to the model and try each one separately. This may not apply, but if
there are monomers in the SAXS model that are related by space group symmetry
in the crystal, I think the MR would never work. Good luck
Dear Kevin
You could try reindexing into P1, then run Phaser and with its solution as
input to Zanuda determine the space group.
Best wishes,
John
Emeritus Professor of Chemistry John R Helliwell DSc_Physics
> On 31 May 2019, at 21:09, Kevin Jude wrote:
>
> Hello community, I wonder if
Thanks Diana - Indexing on strong reflections (STRONG_PIXEL=50 in xds) does
identify C222 as a possibility with the same dimensions as the P222 cell.
This doesn't solve my problem, though, since the centering operation just
replaces the tNCS and doesn't relieve the crowding.
Best wishes
Kevin
--
Wouldn't c222 or c222(1) be included in the Phaser run if all orthorhombic sg's were requested?
Boaz
Boaz Shaanan, Ph.D.
Department of Life Sciences
Ben Gurion University of the Negev
Beer Sheva
Israel
On May 31, 2019 23:37, Diana Tomchick wrote:
Your native Patterson
Your native Patterson indicates pseudo C-centering. Are you sure you don’t have
space group C222(1)?
If your space group is correct, it’s still pseudo C-centered. You should see
that in the intensity-weighted reciprocal lattice.
You could try re-indexing on just the most intense spots to give
Hello community, I wonder if I could solicit advice about a problematic
dataset. I plan to solve the structure by molecular replacement and expect
that the protein is relatively compact, ie not elongated. SAXS data
supports this expectation.
The crystals diffract to 2.6 Å resolution and appear to
