Dear all,
I have a 20 KDa protein that has a single cys residue , about 42
residues from the C-terminal . The protein aggregates during crystallization
setup ( hanging drop vapour diffusion) . What ratio of protein and
mercaptoethanol is to be used to avoid aggregation and help in
hi sreetama,
if ur protein is making aggregates during
crystallization try to add glycerol, EDTA in Ur buffer. R u purifying ur
protein from Gelfiltration column ?is ur protein is coming in proper place?
if every thing is right then u can try DTT and TCEP in ur buffer
