[ccp4bb] weak density for the missing part

Dear Friends, I recently have a partially complete(90%) model that refined to R = 26% and R(free) = 30% at 2.5 A data and looks fine in the 2fo-fc map. This is a complex molecule comprising two proteins and one dna in the AU. Protein 1(285 aa) and dna(15 base pairs) have

Re: [ccp4bb] weak density for the missing part

Hello Raja, Are you sure your smaller protein is in your crystal? Have you run a gel or performed mass spec on dissolved crystals? ho UC Berkeley