Re: [ccp4bb] zinc fingre

2012-04-04 Thread Rajesh kumar
Dear All, Thanks everyone for the suggestions. I did go to library to look up some chapters in books which gave me lot of information about conserved zinc finger domain. My protein has Cx4C and Cx3C and with very large spacing, so I suspect it could be zinc finger like protein (though no

Re: [ccp4bb] zinc fingre

2012-04-03 Thread Debasish Chattopadhyay
] On Behalf Of Pius Padayatti Sent: Tuesday, April 03, 2012 11:51 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] zinc fingre Hi Rajesh, First of all you did the right thing to ask people here about our doubts. There is nothing wrong in asking questions. The board is for asking questions realted

Re: [ccp4bb] zinc fingre

2012-04-03 Thread Peter Hsu
Hi Rajesh, Have you looked at how well conserved these Cys/His residues are? Is the spacing similar to known zinc fingers? Might be good things to consider if you suspect a zinc finger in your protein, of course you probably know this already. Best, Peter

[ccp4bb] zinc fingre

2012-04-03 Thread Rajesh kumar
Dear All, I am trying to crystallize a protein, so far I got no diffraction though I have large crystals.It has few cystines and a histidine near by at N-terminal. I dont have much literature on biochemistry of this protein available in pubmed (5 papers only).Is there a way if I could check

Re: [ccp4bb] zinc fingre

2012-04-03 Thread Debasish Chattopadhyay
Read a book. If you can't find a book then ask the all knowing Google. From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Rajesh kumar Sent: Tuesday, April 03, 2012 10:07 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] zinc fingre Dear All, I am trying to crystallize

Re: [ccp4bb] zinc fingre

2012-04-03 Thread Rajesh kumar
Thanks.. I will. Date: Tue, 3 Apr 2012 15:17:34 + From: debas...@uab.edu Subject: Re: [ccp4bb] zinc fingre To: CCP4BB@JISCMAIL.AC.UK Read a book. If you can’t find a book then ask the all knowing Google. From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf

Re: [ccp4bb] zinc fingre

2012-04-03 Thread Pius Padayatti
Hi Rajesh, First of all you did the right thing to ask people here about our doubts. There is nothing wrong in asking questions. The board is for asking questions realted to crystallography (all aspects). Padayatti On Tue, Apr 3, 2012 at 11:07 AM, Rajesh kumar ccp4...@hotmail.com wrote: Dear