[ccp4bb] 3D Glasses - Vuzix HMD by eDimensional

2008-02-04 Thread Pedro M. Matias
Dear Colleagues, I found out that eDimensional sells the Vuzix HMD 3D glasses, but their stereo drivers only work in windows. I suppose that they should also work under Linux with the Nvidia stereo drivers. However because we must pay by purchase order and bank transfer, they will not honor

Re: [ccp4bb] 3D Glasses - Vuzix HMD by eDimensional

2008-02-04 Thread Andrew Raine
Anastassis Perrakis wrote: While we are on the subject, does anyone in general have working in their labs a stereo-3D solution that does not require CRT monitors but works on LCD and preferably with Linux or OSX ? (any windows hints are welcome as well). Yes indeed. We have a 20 one of

Re: [ccp4bb] 3D Glasses - Vuzix HMD by eDimensional

2008-02-04 Thread Pedro M. Matias
At USD 7000 it's not exactly the cheap solution I was looking for... At 10:46 04-02-2008, Andrew Raine wrote: Anastassis Perrakis wrote: While we are on the subject, does anyone in general have working in their labs a stereo-3D solution that does not require CRT monitors but works on LCD and

Re: [ccp4bb] 3D Glasses - Vuzix HMD by eDimensional

2008-02-04 Thread P Hubbard
Hi Andrew, Just like the commercial systems, the glass is the only special piece of kit (which can be bought separately). The LCD monitors are just set up to display either left or right channel. If you ask me, I think these companies are just a rip off! Paul Date: Mon, 4 Feb 2008 11:24:32

[ccp4bb] Still cannot read .mtz + another ?

2008-02-04 Thread C.Ainsley Davis
Hey all I have checked everything that was mentioned here. I dont have PHENIX installed ( I am using the current version from http://diablo/ucsc.edu/~wgscott/debian/deb/ccp4/) I checked the setup file and its set to 1 Any other ideas? Next question I would like to install the newest

Re: [ccp4bb] 3D Glasses - Vuzix HMD by eDimensional

2008-02-04 Thread Harry Powell
Okay, I was wrong. On two points... What I'd forgotten is that LCD displays produce polarized light (and so do TFT displays, for that matter), so you don't need a sheet of Polaroid to polarize the light from the vertical display. The half-silvered mirror is there (of course, I hear the

[ccp4bb] Synchrotrons and Lasers for Structural Systems Biology

2008-02-04 Thread Victor Lamzin
We would like to announce the Symposium on Synchrotrons and Lasers for Structural Systems Biology to be held on 16th April 2008 at the premises of the EMBL/DESY in Hamburg, Germany. International experts in the field of the use of synchrotron radiation in biological research will present their

Re: [ccp4bb] WG: [ccp4bb] 3D Glasses - Vuzix HMD by eDimensional

2008-02-04 Thread Jeroen Mesters
Hi Gregor, I think this LCD monitor is not useful since the refresh rate is only 75 Hz, thus in stereo 2 x 37.5 Hz and that is going to give a big headache. I know Samsung is working on a 100 Hz LCD-TV. Nevertheless, the goal is not for stereo but to suppress the afterglow effects again. They

Re: [ccp4bb] Still cannot read .mtz + another ?

2008-02-04 Thread C.Ainsley Davis
Peter and Miguel, thanks for the help with Refmac, it appears to be working correctly! still issues with PHASER though.. thanks a bunch! Ainsley P.J.Briggs wrote: Dear Ainsley I'm not sure which file you took from Garib's page, however the CCP4i install options (under the System

Re: [ccp4bb] an over refined structure

2008-02-04 Thread Anastassis Perrakis
Hi - I don't think there is something necessarily wrong with the values you report. A few questions to see *if* something is wrong are: - as you wrote to Tim you have NCS: do you use NCS restraints ? - what is the resolution / B factor of the data ? - have the data been checked for twining

Re: [ccp4bb] an over refined structure

2008-02-04 Thread Tim Gruene
I would agree that the difference is suspiciously high. I. Tickle and others have published analytical expressions for how to estimate the ratio between R and Rfree, just google for tickle rfree to find the references. You easily achieve a large difference by adding too many waters which just

Re: [ccp4bb] 3D Glasses - Vuzix HMD by eDimensional

2008-02-04 Thread Warren DeLano
Pedro, The DV920 works even with frame-sequential stereo, but with a native resolution of 640x480, they aren't useful for real work. Plus, they're not all that comfortable -- I returned them after a couple of week. According to the Vuzix rep I spoke to on the phone, we are still years away from

Re: [ccp4bb] an over refined structure

2008-02-04 Thread Ian Tickle
Hi Sun Tang Unfortunately there's no such thing as a fixed value for the maximum acceptable Rfree-Rwork difference that applies in all circumstances, because the 'normal' difference depends on a number of factors, mainly the observation/parameter ratio, which depends in turn on the resolution

Re: [ccp4bb] an over refined structure

2008-02-04 Thread Sun Tang
Hi Tim, Thank you for your and information and suggestions. There are two indepdent molecules in the asymmetric unit and one molecule does not have very good density, especially in the N-terminus. Do you think that I should remove the region in the refinement? Best, Sun Tim Gruene [EMAIL

Re: [ccp4bb] 3D Glasses - Vuzix HMD by eDimensional

2008-02-04 Thread Pedro M. Matias
Hi, Jeroen The Vuzix VR920 should provide a cheap 3D stereo alternative to CRT monitors, because it contains two small LCD screens - if one displays a right eye view and the other a left eye view we'd have a situation similar to older display systems, with side-by-side stereo and a 3D viewer

Re: [ccp4bb] 3D Glasses - Vuzix HMD by eDimensional

2008-02-04 Thread P Hubbard
Hi all, There's a pretty good description of how it works, and how to make one yourself here: http://forums.nvidia.com/index.php?showtopic=32547 I've never used one myself, and I personally feel that LCD stereo with a large f.o.v. on a single flat panel should be available soon (I think they

Re: [ccp4bb] 3D Glasses - Vuzix HMD by eDimensional

2008-02-04 Thread Harry Powell
Hi Just looking at the diagrams, I don't think the glass is half-silvered - it looks like a large sheet of Polaroidâ„¢. It only needs to polarize the transmitted light from the vertically oriented monitor, since the reflected light from the interface between two materials (at least one of

Re: [ccp4bb] 3D Glasses - Vuzix HMD by eDimensional

2008-02-04 Thread esko . oksanen
Hi, We tested a Planar 17 stereo monitor a year or so ago on our Macs (with the image flipping card) and the problem was that although the stereo effect was nice, we had to use side-by-side and an extended desktop stereo in eg. PyMol to get the left and right eye images to the right

[ccp4bb] an over refined structure

2008-02-04 Thread Sun Tang
Hello All, I refined a structure with Refmac in CCP4i and the R/Rfree is 0.215/0.277. The difference between R and Rfree is too much even though I used 0.01 for weighting term in the refinement (the default value is 0.3). The RMSD for bond length and bond angle is 0.016 A and 1.7 degree.

[ccp4bb] an over refined structure

2008-02-04 Thread George M. Sheldrick
Dear Sun, If we take Ian's formula for the ratio of R(free) to R(work) from his paper Acta D56 (2000) 442-450 and make some reasonable approximations, we can reformulate it as: R(free)/R(work) = sqrt[(1+Q)/(1-Q)] with Q = 0.025pd^3(1-s) where s is the fractional solvent content, d is the

Re: [ccp4bb] an over refined structure

2008-02-04 Thread Sun Tang
Hi Ian, Thank you very much for your detailed information. I checked the effect of weighter term (wa) in CCP4i for the R/Rfree. When I used wa=0.01 , the value is 0.225/0.277 FOM =0.799. The values changed to 0.204/0.269 (FOM=0.806) for wa= 0.05, 0.195/0.268 (FOM=0.807) for wa=0.1 and

Re: [ccp4bb] an over refined structure

2008-02-04 Thread Sun Tang
Hi Anastassis, Thank you very much for your suggestions. I answered the questions as follows. I used NCS before rigid body refinement. After that I did not put NCS restraints in the restrained refinement and TLS+restrained refinement because it raised the R/Rfree quite a lot. The resolution is

Re: [ccp4bb] Question about strange MR solution

2008-02-04 Thread Marius Schmidt
Dear Michelle, this is not strange at all. You simply have a MR solution that refers to a different origin. You cannot display the original model plus the MR solution in the same coordinate system (with respect to the same origin). Of course they clash. Greetings Marius Dear all, I refined

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2008-02-04 Thread Marius Schmidt
Dear colleagues, someone out there who has installed XtalView with the new SUSE Linux 10.3. When launching Xfit, there is an error message xfit: xcb_io.c: _XAllocID: Assertion `!(dpy - flags etc. etc. when googeling for this error, it is well reported to occur for the newest Linux systems.

[ccp4bb] Question about strange MR solution

2008-02-04 Thread Michele Lunelli
Dear all, I refined a protein structure in the space group P6(1)22, with one copy in the asymmetric unit, resolution ~1.8 A, Rwork=0.20, Rfree=0.22. Then I tried to feed Phaser (version 1.3.3) with this structure. It found quickly a very prominent solution, but the first euler angle is 180

[ccp4bb] His tag

2008-02-04 Thread Yanming Zhang
Hi All, Maybe, I should not have asked this question: Can anybody give me the hints (or point to the references) on the impact of His tag on crystallization experiments. In perticular: 1, With or without His tag, which one is better for crystallization? 2, If I successfully crystallized

Re: [ccp4bb] 3D Glasses - Vuzix HMD by eDimensional

2008-02-04 Thread David M Shechner
Well, it's not exactly a solution that could be quickly implemented in the short term, but given the rise of open-source crystallography software, I wonder if modules could be written to let the viewer see 3D objects on a 2D display using head tracking. You'd have to nod your head slightly

Re: [ccp4bb] His tag

2008-02-04 Thread Artem Evdokimov
Yangming, This topic has been discussed before - basically there are no easily discernable trends - some proteins crystallize better with a tag, others - without, and yet others - don't care whether the tag is there or not. I tend to try either, just to see what works better (time and effort

Re: [ccp4bb] His tag

2008-02-04 Thread Bostjan Kobe
Dear Yanming Lots of proteins have been crystallized with His-tags on. However, in general one would assume that a flexible tag could have a negative effect. I am not aware of a systematic comparison of crystallization of tagged and untagged proteins, but the following paper is relevant to this

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2008-02-04 Thread Tim Gruene
Could you find out whether this error persists across different distributions? Would one solution be to use MIFit instead of xtalview? MIFit is the official successor of the now unsupported xtalview and as far as I know also available for free for academic users. Tim -- Tim Gruene Institut