Re: [ccp4bb] combine Se-SAD phase with Model-phase

2008-05-08 Thread Eleanor Dodson
This is only a suggestion but sometimes it helps. First idea - try as hard as you can to extend the native data - at 3.6A resolution all model building is extremely hard! To work with what you have I would do this - others may have different ideas. Combine both data sets into one mtz file,

Re: [ccp4bb] combine Se-SAD phase with Model-phase

2008-05-08 Thread Clemens Vonrhein
On Thu, May 08, 2008 at 07:26:40AM +0530, Raja Dey wrote: 3. Run SHARP/AutoSHARP with Se Just run autoSHARP in SIRAS mode: you have a native and a derivative (Se). autoSHARP is clever enough to then assign those special Se-S atoms to you derivative, see the explanation at

[ccp4bb] Ferritin crystallisation

2008-05-08 Thread Clemens Grimm
Dear all, scanning the literature, it seems that horse spleen Ferritin has so far been crystallised only in presence of Cadmium ions. Is this correct? Does anybody know of conditions lacking Cd? Clemens

[ccp4bb] Post-Doctoral Opportunity in Oulu, Finland

2008-05-08 Thread Petri Kursula
Post-Doctoral Opportunity in Oulu, Finland A post-doctoral opportunity, funded by the Sigrid Juselius Foundation, exists in our group for an initial period of approximately 1 year, with possible extension. The work will involve structural studies on proteins important for the function of

[ccp4bb] stereo with Nvidia Quadro

2008-05-08 Thread Tim Gruene
Good day, I have been trying to enable stereo mode on a PC (SuSE10.1) with an NVIDIA NV37GL [Quadro FX 330/Quadro NVS280] graphics card (according to Xorg.0.log). (--) PCI:*(5:0:0) nVidia Corporation NV37GL [Quadro FX 330/Quadro NVS280] rev 162, Mem @ 0xdd00/24, 0xc000/28,

Re: [ccp4bb] stereo with Nvidia Quadro

2008-05-08 Thread David J. Schuller
The nVidia cards used for stereo are the Quadro FX series. The Quadro NVS series are for running multiple monitors, but not fancy 3D graphics. You can explore the manufacturer's web site for details. - === With the single

Re: [ccp4bb] stereo with Nvidia Quadro

2008-05-08 Thread Tim Gruene
Dear David, thank you for your answer. Do you (or anyone on the list) know if any FX-card would work with NuVision 60GX (like FX370, FX570), or only the dear ones (FX1400 and above)? Tim -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A

Re: [ccp4bb] stereo with Nvidia Quadro

2008-05-08 Thread David Roberts
Just check out the cards on the back - it should have the small round port that a 60GX plug would fit into. The NuVision site has a list of cards that are compatible with their emitters. I don't remember them all right off, but it's fairly straightforward. By the way, I buy all my video

[ccp4bb] 2008 Gordon Research Conference on Diffraction Methods in Structural Biology

2008-05-08 Thread Elspeth Garman
There is still time to apply to attend this GRC, relevant to researchers interested in Methods Development - details below! The official closing date is 22nd June, but places are limited to a total of 125. Best wishes Elspeth Garman and Andrew Leslie Gordon Research Conference on Diffraction

Re: [ccp4bb] HKL2000 and gcc4 - redux

2008-05-08 Thread James Stroud
On May 7, 2008, at 3:07 PM, Chris Waddling wrote: so even temporarily putting a library where it doesn't belong Actually, this is what /usr/lib is for (except for the doesn't part). According to the Filesystem Hierarchy Standard 4.7 regarding the general requirements and limitations of

Re: [ccp4bb] stereo with Nvidia Quadro

2008-05-08 Thread Eric Bennett
Dear David, thank you for your answer. Do you (or anyone on the list) know if any FX-card would work with NuVision 60GX (like FX370, FX570), or only the dear ones (FX1400 and above)? Nvidia has been progressively dropping support from their lower-end cards. For example the older FX1100 has

Re: [ccp4bb] stereo with Nvidia Quadro

2008-05-08 Thread Sabuj Pattanayek
The 3500 series is moderately expensive. If you are on a budget maybe you could snag an older used card online somewhere. You can still buy new 1400's (OEM) for less than $160. Go to compuvest.com and search for quadro 1400.

[ccp4bb] How to generate two molecules of proteins and two chains of RNA in CNS 1.2

2008-05-08 Thread Sun Tang
Dear All, When I used CNS 1.2 to generate the pdb file for two molecules of proteins and two chains of RNA (with different chain ids in format of CCP4), only one RNA chain is generated. Can anyone tell me how to correctly generate the files used for refinement with CNS 1.2? I