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phew - and people claim, macs were user-friendly. On Linux you just
add '-static' (at least for command line tools as the shelx programs)
and make the sane assumption that the kernel is less than say 10 years
old...
Tim
On 02/26/2013 04:50 PM,
*Early bird registration deadline Friday 1st March 2013!*
*REGISTER NOW!!!*
*11th International Conference on Biological Synchrotron Radiation (BSR)*
*Hamburg, Germany*
*8th - 11th September 2013*
The conference aims to bring together scientists involved in the
methodical developments on
I am trying to compile refmac from source on a machine running Ubuntu
12.04. In a nutshell, after some troubleshooting I end up with
executable that generates a segmentation fault. Log-file states that
CCP4 library signal ccp4_parser:Failed to open external command
file (Success)
Postdoc Position in Structural Biology of Membrane Proteins
A postdoc position is immediately available at the Skirball Institute of
Biomolecular Medicine, New York University School of Medicine, to study the
mechanism of membrane transporters using X-ray crystallography in combination
with
Side note,
Has anyone tried this in a non-E. coli expression system?
Thanks,
Lori
Lori Horton, Ph.D.
Koehler Lab
lori.b.hor...@uth.tmc.edumailto:lori.b.hor...@uth.tmc.edumailto:lori.b.hor...@uth.tmc.edu
713-500-5451
From: CCP4 bulletin board
Dear all
Out of curiosity, are cell free expression systems using E. coli or insect cell
extracts useful for membrane protein expression-crystallization? What are the
costs associated with these systems?
Thank you.
Theresa
I've just read that today (even if it is NMR).
http://www.nature.com/nmeth/journal/v9/n8/full/nmeth.2033.html
/E. coli/ or insects cells are so easy to grow that you should be able
to do your own cell extrats easily.
Xavier
Le 27/02/2013 19:25, Theresa Hsu a écrit :
Dear all
Out of
Applications are invited for a postdoctoral position in the Washington
University at St. Louis. The NIH supported research will focus on the structure and function of intramembrane enzymes. Studies will use multidisciplinary approaches including macromolecular X-ray crystallography,
Posted on behalf of Ann West:
Job Opening for Protein Production Core Facility Director. The
Department of Chemistry and Biochemistry (http://chem.ou.edu) at the
University of Oklahoma, Norman Campus (http://www.ou.edu) invites
applications for a full-time staff position as Director of the
The underlying issue is that the implementation of OS X dynamic linking is
dependent system architecture. As I understand it, OS X 10.5.8 has a 32 bit
kernel, so its dynamic linking is not forward-compatible with 64 bit OS X
versions, for which SHELX-2013 seems to built.
Also, to be fair, it's
After consulting several Mac users, I decided that there were very few
32-bit Mac operating systems still in use by crystallographers, so (as
stated on the new SHELX homepage) I am only providing a 64-bit
executables for Macs. The Mac executables do not run on old Macs with
PPC processors
Dear users,
Is it mandatory to use the same reflections for
Rfree calculations of a ligand bound data as that
of its native?
Thank you
With Regards
Kavya
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Dear Kavya,
It is not so. It is not
mandatory.
best,
Sekar
Dear users,
Is it mandatory to use the same reflections for
Rfree calculations of a ligand bound data as that
of its native?
Thank you
With Regards
Kavya
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Dear CCP4 members
I wish to engineer a disulfide bond at the dimer interface of a protein I am
working with. Does anyone know of any available software to assist with this?
Best
Careina
Hello,
I think this depends on the type of problem you are facing:
if the 2 crystals are not isomorphous then you cannot have the same
R-free sets;
if the 2 crystals are isomorphous then either you do not worry about
keeping the same R-free set (but then the starting structure must be
Although it is not mandatory, I think it would be a very good idea,
especially if you have the exact same spacegroup and the native and
ligand-bound forms of your protein are essentially the same.
If you do not use the same reflections, you are actually not reporting an
independent (free)
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