Re: [ccp4bb] SHELX-2013 release and homepage

2013-02-27 Thread Tim Gruene
-BEGIN PGP SIGNED MESSAGE- Hash: SHA1 phew - and people claim, macs were user-friendly. On Linux you just add '-static' (at least for command line tools as the shelx programs) and make the sane assumption that the kernel is less than say 10 years old... Tim On 02/26/2013 04:50 PM,

[ccp4bb] Early Bird Deadline 1st March! 11th International Conference on Biological Synchrotron Radiation (BSR), Hamburg, Germany, 8-11 Sept 2013

2013-02-27 Thread Rosemary Wilson
*Early bird registration deadline Friday 1st March 2013!* *REGISTER NOW!!!* *11th International Conference on Biological Synchrotron Radiation (BSR)* *Hamburg, Germany* *8th - 11th September 2013* The conference aims to bring together scientists involved in the methodical developments on

[ccp4bb] compiling refmac5 on Ubuntu 12.04

2013-02-27 Thread Ed Pozharski
I am trying to compile refmac from source on a machine running Ubuntu 12.04. In a nutshell, after some troubleshooting I end up with executable that generates a segmentation fault. Log-file states that CCP4 library signal ccp4_parser:Failed to open external command file (Success)

[ccp4bb] Postdoc Position in Structural Biology of Membrane Proteins

2013-02-27 Thread Wang, Da-Neng
Postdoc Position in Structural Biology of Membrane Proteins A postdoc position is immediately available at the Skirball Institute of Biomolecular Medicine, New York University School of Medicine, to study the mechanism of membrane transporters using X-ray crystallography in combination with

Re: [ccp4bb] Additives to bacterial cultures to improve solubility

2013-02-27 Thread Horton, Lori B
Side note, Has anyone tried this in a non-E. coli expression system? Thanks, Lori Lori Horton, Ph.D. Koehler Lab lori.b.hor...@uth.tmc.edumailto:lori.b.hor...@uth.tmc.edumailto:lori.b.hor...@uth.tmc.edu 713-500-5451 From: CCP4 bulletin board

[ccp4bb] Cell free expression

2013-02-27 Thread Theresa Hsu
Dear all Out of curiosity, are cell free expression systems using E. coli or insect cell extracts useful for membrane protein expression-crystallization? What are the costs associated with these systems? Thank you. Theresa

Re: [ccp4bb] Cell free expression

2013-02-27 Thread Xavier Brazzolotto
I've just read that today (even if it is NMR). http://www.nature.com/nmeth/journal/v9/n8/full/nmeth.2033.html /E. coli/ or insects cells are so easy to grow that you should be able to do your own cell extrats easily. Xavier Le 27/02/2013 19:25, Theresa Hsu a écrit : Dear all Out of

[ccp4bb] Postdoc Position

2013-02-27 Thread Li, Weikai
Applications are invited for a postdoctoral position in the Washington University at St. Louis. The NIH supported research will focus on the structure and function of intramembrane enzymes. Studies will use multidisciplinary approaches including macromolecular X-ray crystallography,

[ccp4bb] Protein Production Core Director - Job Opening - U or Oklahoma

2013-02-27 Thread Leonard Thomas
Posted on behalf of Ann West: Job Opening for Protein Production Core Facility Director. The Department of Chemistry and Biochemistry (http://chem.ou.edu) at the University of Oklahoma, Norman Campus (http://www.ou.edu) invites applications for a full-time staff position as Director of the

Re: [ccp4bb] SHELX-2013 release and homepage

2013-02-27 Thread James Stroud
The underlying issue is that the implementation of OS X dynamic linking is dependent system architecture. As I understand it, OS X 10.5.8 has a 32 bit kernel, so its dynamic linking is not forward-compatible with 64 bit OS X versions, for which SHELX-2013 seems to built. Also, to be fair, it's

Re: [ccp4bb] SHELX-2013 release and homepage

2013-02-27 Thread George Sheldrick
After consulting several Mac users, I decided that there were very few 32-bit Mac operating systems still in use by crystallographers, so (as stated on the new SHELX homepage) I am only providing a 64-bit executables for Macs. The Mac executables do not run on old Macs with PPC processors

[ccp4bb] Rfree flag

2013-02-27 Thread Kavyashree Manjunath
Dear users, Is it mandatory to use the same reflections for Rfree calculations of a ligand bound data as that of its native? Thank you With Regards Kavya -- This message has been scanned for viruses and dangerous content by MailScanner, and is believed to be clean.

Re: [ccp4bb] Rfree flag

2013-02-27 Thread Prof. K. Sekar
Dear Kavya, It is not so. It is not mandatory. best, Sekar Dear users, Is it mandatory to use the same reflections for Rfree calculations of a ligand bound data as that of its native? Thank you With Regards Kavya -- This message has been scanned for viruses and dangerous

[ccp4bb] disulfide engineering

2013-02-27 Thread Careina Edgooms
Dear CCP4 members I wish to engineer a disulfide bond at the dimer interface of a protein I am working with. Does anyone know of any available software to assist with this? Best Careina

Re: [ccp4bb] Rfree flag

2013-02-27 Thread vellieux
Hello, I think this depends on the type of problem you are facing: if the 2 crystals are not isomorphous then you cannot have the same R-free sets; if the 2 crystals are isomorphous then either you do not worry about keeping the same R-free set (but then the starting structure must be

Re: [ccp4bb] Rfree flag

2013-02-27 Thread Folmer Fredslund
Although it is not mandatory, I think it would be a very good idea, especially if you have the exact same spacegroup and the native and ligand-bound forms of your protein are essentially the same. If you do not use the same reflections, you are actually not reporting an independent (free)