Proprietary
Commenting on Patrick’s message:
(snip) might also consider that PEGs are notorious for impurities of various
types; perhaps the Fluka reagent has less of an impurity that is particularly
vexing for your specific
protein.
Of course, I suppose it might have more of a particular
Good luck Mr. Hyde!
jon
Von: CCP4 bulletin board Im Auftrag von Correy, Galen
Gesendet: Montag, 22. März 2021 18:01
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [ccp4bb] Fluka-branded PEG 3000
Dear all,
I'm wondering if anyone could spare 50-100 g of Fluka branded PEG 3000 (see
attached image)?
We
If you even still have a few milliliters of the original PEG solution, you
could simply use that for mixing with the protein in the drop and use a
different PEG solution for the reservoir, thereby ensuring a mother liquor that
could either provide (or remove) a missing impurity.
Diana
Dear all,
We are currently seeking a crystallographer to join the Biomolecular
Structure group at our research facility (Granta Park, Cambridge, UK), to
work on GPCR structure determination to enable drug discovery efforts.
The successful candidate is expected to work in the following areas:
Dear Saurabh,
It's well known that good-looking crystals can have poor internal order,
and therefore yield poor diffraction, and vice versa. As others have
pointed out, it's crucial to check the quality of diffraction at room
temperature (RT).
Just to add a bit to that:
There's a nice book that
Hi Saurabh
Of course you should use "rMMS" microseeding. See below
I hope it works !
Best wishes, Patrick
-- Forwarded message -
From: Patrick Shaw Stewart
Date: Mon, Sep 9, 2019 at 10:12 AM
Subject: Re: [ccp4bb] Optimization from needle shaped crystals
To: David Briggs
Cc:
Hello fellow colleagues
Hope you are all well while the pandemics persists. I just wonder if anyone
may have an idea what this density (looking like a pentagon) might be. The
data was collected to 1.8 A and crystal was grown in Bis-tris + PEG3350.
Imidazole residual? Nucleotide (the protein
Dear all,
We have a new opening for a Post Doc position to join the ID29 upgrade
project at the ESRF.
ID29 is currently being upgraded in a new serial crystallography beamline
that can fully profit from the ESRF Extremely Brilliant Source. We are
looking for someone with strong motivation in
The ring looks too big to be imidazole or a nucleotide or a carbohydrate, so
it’s probably mainly water molecules.
Perhaps partially replaced by PEG to explain the density between them (i.e.
water molecules in most copies of the protein and PEG in some other copies).
I’ve seen horse-shoe shaped
Could be 5 water molecules. Pentagons of water molecules are common in high
resolution structures. Add 5 waters and see if the inter-water distances are
appropriate for hydrogen bonding (2.5-3.2 A).
From: CCP4 bulletin board on behalf of Sam Tang
Date: Monday, March 22, 2021 at 9:00 AM
To:
I don’t think we have any of that PEG lying around (sorry), but you might also
consider that PEGs are notorious for impurities of various types; perhaps the
Fluka reagent has less of an impurity that is particularly vexing for your
specific protein.
Of course, I suppose it might have more of a
Definitely water pentamer, no doubt at all ;-0
Cheers, Jon.C.
Sent from ProtonMail mobile
Original Message
On 22 Mar 2021, 14:16, Mark J. van Raaij wrote:
> The ring looks too big to be imidazole or a nucleotide or a carbohydrate, so
> it’s probably mainly water molecules.
>
Dear Saurabh
I would like to share something that worked for me. I was in a similar
situation, though my crystals weren't as beautiful as yours are. I followed
Alexander McPherson's Crystallization of Biological Macromolecules and what
ultimately worked is as follows:
I did something called
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