Re: [ccp4bb] How to calculate experimental residual electron density map

2023-07-24 Thread Eleanor Dodson
You would hope it would show some hydrogen positions.. Eleanor On Mon, 24 Jul 2023 at 13:47, Ankur Kumar Singh wrote: > Dear Prof. Eleanor Dodson, > Thank you for the response. I will try generating difference map as told > and will update. > > - > Regards, > Ankur K Singh > Ph.D. Student >

Re: [ccp4bb] How to calculate experimental residual electron density map

2023-07-24 Thread Eleanor Dodson
hy not calculate structure factors without hydrogen atoms and look at the difference map? Eleanor On Mon, 24 Jul 2023 at 12:36, Ankur Kumar Singh < a8e375e16ee1-dmarc-requ...@jiscmail.ac.uk> wrote: > Dear Members, > I am trying to generate an experimental residual electron density map for >

Re: [ccp4bb] How to calculate experimental residual electron density map

2023-07-24 Thread Ankur Kumar Singh
Dear Prof. Eleanor Dodson, Thank you for the response. I will try generating difference map as told and will update. - Regards, Ankur K Singh Ph.D. Student From: Eleanor Dodson Sent: Monday, July 24, 2023 5:31 PM To: Ankur Kumar Singh Cc:

[ccp4bb] How to calculate experimental residual electron density map

2023-07-24 Thread Ankur Kumar Singh
Dear Members, I am trying to generate an experimental residual electron density map for a protein structure resolved at 1.1 Angstrom. One way to do this is by refining the structure using an aspherical model of charge distribution (Multipole density proposed by Hansen and Coppens) in MoPro.

[ccp4bb] 1990s-style stereo viewer

2023-07-24 Thread Harry Powell
Hi folks I was wondering if anyone has a photo of the old-style head-mounted stereo viewers that used to be used to see 3D images from wall-eyed stereo pairs? I don’t mean the ones that were used to view the side-by-side stereo images that once appeared in printed journals, but the ones that

Re: [ccp4bb] 1990s-style stereo viewer

2023-07-24 Thread Harry Powell
Hi Alastair YES! Those are the ones. Many thanks Harry > On 24 Jul 2023, at 16:46, Alastair MC EWEN wrote: > > Hi Harry, > > Are these the ones you mean? > > Best, > Alastair > > ~ > Alastair McEwen, PhD > Integrated Structural Biology Platform > IGBMC > 1 rue Laurent Fries > 67404

Re: [ccp4bb] 1990s-style stereo viewer

2023-07-24 Thread Harry Powell
Can’t have been a PS300 in that case! I’ve since been reminded that they were made of wood (the stereo viewers, not the Evans and Sutherland. But I’m probably wrong about that as well…) Harry > On 24 Jul 2023, at 16:03, Wim Burmeister wrote: > > Hi, > the E PS300 already used LCD-based

Re: [ccp4bb] 1990s-style stereo viewer

2023-07-24 Thread Wim Burmeister
Hi, the E PS300 already used LCD-based shutter glasses connected with a wire although awfully flickering with a switching frequency in the 8 Hz range! Wim - Mail original - De: "Harry Powell" <193323b1e616-dmarc-requ...@jiscmail.ac.uk> À: "CCP4BB" Envoyé: Lundi 24 Juillet 2023

Re: [ccp4bb] How to calculate experimental residual electron density map

2023-07-24 Thread Tim Gruene
Dear Amkur K Singh, every refinement program (buster, refmac5, phenix.refine, SHELXL) produces coefficients for the electron density map, that you name "2Fmo-dFc", as well es the residual electron density map. That's named 'difference map'. Unless I misunderstand, that is what you are looking

Re: [ccp4bb] 1990s-style stereo viewer

2023-07-24 Thread Goldman, Adrian
Yes but this is just classic side by side stereo. I had one until quite recently. My memory of the ps300 is that it was fullscreen, not side by side with the display synced as another poster said. I also feel that the ps300 didn’t flicker because it was a raster display so no matter how many

[ccp4bb] PNCC Screening Access Mechanism

2023-07-24 Thread Kjirsten Wheeler
Hello CryoEM Researchers, Pacific Northwest CryoEM Center is offering a mechanism developed to help laboratories with limited or no cryo-EM resources enter the field of cryo-EM. PNCC's "Screening Access Mechanism" will provide users on-site access to state-of-the-art cryo-EM resources and