Re: [ccp4bb] journals with on-hold policy

2007-02-19 Thread Eric Toth
I think Martha Stewart's magazine has fairly liberal data deposition policies as well. _ Eric A. Toth, Ph.D. Assistant Professor Department of Biochemistry and Molecular Biology Marlene and Stewart Greenebaum Cancer Center University of Maryland

Re: [ccp4bb] Protein expression in Minimal media (M9)

2007-04-19 Thread Eric Toth
I had a similar situation, i.e. the cells wouldn't grow past an OD of 0.1 on minimal media, but in my case I did get protein expression. The way I got around it was as follows. I grew the cells up in minimal media plus the amino acids that suppress methionine biosynthesis, plus L-methionine.