Re: [ccp4bb] High resolution + low resolution data sets from one crystal

2011-05-30 Thread Machius, Mischa Christian
Dear Pengfei, Indeed, for HDR crystallography (high dynamic range) simply (integrate and) scale both batches of images at the same time in HKL2000. Depending on the space group, make sure indexing is consistent between the two datasets. Scaling then takes care of combining the two batches.

Re: [ccp4bb] Nanodrop versus Nanophotomter Pearl versus good old Bradford.

2011-06-16 Thread Machius, Mischa Christian
With respect to the Edelhoch method and the ProtParam server, I would strongly recommend determining extinction coefficients experimentally and not rely on the ProtParam values. The reason is that the underlying extinction coefficients in the formula used by ProtParam and referenced there are

Re: [ccp4bb] Nanodrop versus Nanophotomter Pearl versus good old Bradford.

2011-06-16 Thread Machius, Mischa Christian
, Machius, Mischa Christian wrote: With respect to the Edelhoch method and the ProtParam server, I would strongly recommend determining extinction coefficients experimentally and not rely on the ProtParam values. The reason is that the underlying extinction coefficients in the formula used

Re: [ccp4bb] Nanodrop versus Nanophotomter Pearl versus good old Bradford.

2011-06-16 Thread Machius, Mischa Christian
for the Extinction Coefficient measurement (which requires knowledge of protein concentration)? Let me guess, Bradford? Protein evaporation and weighing? Alex On Jun 16, 2011, at 4:22 PM, Machius, Mischa Christian wrote: With respect to the Edelhoch method and the ProtParam server, I would strongly

Re: [ccp4bb] Nanodrop versus Nanophotomter Pearl versus good old Bradford.

2011-06-16 Thread Machius, Mischa Christian
On Jun 16, 2011, at 8:30 PM, Dima Klenchin wrote: You recommended determining extinction coefficients experimentally. How is plugging number of specific residues into a formula constitute experimental determination? That is a deeply philosophical question! Eventually, you'll be plugging in